医药导报
醫藥導報
의약도보
HERALD OF MEDICINE
2014年
9期
1140-1143
,共4页
潘晓丽%熊永爱%谭玉柱%向晖
潘曉麗%熊永愛%譚玉柱%嚮暉
반효려%웅영애%담옥주%향휘
马齿苋总黄酮%肝硬化%转化生长因子
馬齒莧總黃酮%肝硬化%轉化生長因子
마치현총황동%간경화%전화생장인자
Total flavones from portulaca%Liver cirrhosis%Transforming growth factors
目的：观察马齿苋总黄酮对肝纤维化大鼠转化生长因子( TGF)-β1基因及其蛋白表达的影响,探讨马齿苋总黄酮对肝纤维化的治疗作用机制。方法 SD大鼠48只,随机分为正常对照组、模型对照组、复方甘草酸苷组、马齿苋总黄酮组,各12只。除正常对照组外,其余各组大鼠腹腔注射四氯化碳( CCl4)溶液2 mL · kg-1· d-1复制肝纤维化模型。复方甘草酸苷组灌胃复方甘草酸苷溶液15.75 mg·kg-1,马齿苋总黄酮组灌胃马齿苋总黄酮溶液35.6 mg·kg-1,正常对照组和模型对照组灌胃等体积纯净水。30 d后麻醉处死动物剖取肝脏,实时-聚合酶链反应( RT- PCR)和Western-Blot法检测肝脏细胞TGF-β1基因及蛋白表达。结果正常对照组、模型对照组、复方甘草酸苷组、马齿苋总黄酮组大鼠肝脏细胞中TGF-β1基因的相对表达量分别为0.725±0.130,7.493±1.410,3.016±1.240,2.668±1.150。马齿苋总黄酮可显著下调TGF-β1基因(P<0.01)及TGF-β1蛋白(P<0.01)的表达。结论马齿苋总黄酮可通过下调大鼠肝纤维化细胞TGF-β1基因及蛋白表达有效治疗肝细胞纤维化病变。
目的：觀察馬齒莧總黃酮對肝纖維化大鼠轉化生長因子( TGF)-β1基因及其蛋白錶達的影響,探討馬齒莧總黃酮對肝纖維化的治療作用機製。方法 SD大鼠48隻,隨機分為正常對照組、模型對照組、複方甘草痠苷組、馬齒莧總黃酮組,各12隻。除正常對照組外,其餘各組大鼠腹腔註射四氯化碳( CCl4)溶液2 mL · kg-1· d-1複製肝纖維化模型。複方甘草痠苷組灌胃複方甘草痠苷溶液15.75 mg·kg-1,馬齒莧總黃酮組灌胃馬齒莧總黃酮溶液35.6 mg·kg-1,正常對照組和模型對照組灌胃等體積純淨水。30 d後痳醉處死動物剖取肝髒,實時-聚閤酶鏈反應( RT- PCR)和Western-Blot法檢測肝髒細胞TGF-β1基因及蛋白錶達。結果正常對照組、模型對照組、複方甘草痠苷組、馬齒莧總黃酮組大鼠肝髒細胞中TGF-β1基因的相對錶達量分彆為0.725±0.130,7.493±1.410,3.016±1.240,2.668±1.150。馬齒莧總黃酮可顯著下調TGF-β1基因(P<0.01)及TGF-β1蛋白(P<0.01)的錶達。結論馬齒莧總黃酮可通過下調大鼠肝纖維化細胞TGF-β1基因及蛋白錶達有效治療肝細胞纖維化病變。
목적：관찰마치현총황동대간섬유화대서전화생장인자( TGF)-β1기인급기단백표체적영향,탐토마치현총황동대간섬유화적치료작용궤제。방법 SD대서48지,수궤분위정상대조조、모형대조조、복방감초산감조、마치현총황동조,각12지。제정상대조조외,기여각조대서복강주사사록화탄( CCl4)용액2 mL · kg-1· d-1복제간섬유화모형。복방감초산감조관위복방감초산감용액15.75 mg·kg-1,마치현총황동조관위마치현총황동용액35.6 mg·kg-1,정상대조조화모형대조조관위등체적순정수。30 d후마취처사동물부취간장,실시-취합매련반응( RT- PCR)화Western-Blot법검측간장세포TGF-β1기인급단백표체。결과정상대조조、모형대조조、복방감초산감조、마치현총황동조대서간장세포중TGF-β1기인적상대표체량분별위0.725±0.130,7.493±1.410,3.016±1.240,2.668±1.150。마치현총황동가현저하조TGF-β1기인(P<0.01)급TGF-β1단백(P<0.01)적표체。결론마치현총황동가통과하조대서간섬유화세포TGF-β1기인급단백표체유효치료간세포섬유화병변。
Objective To explore the effects of total flavonoids from portulaca against liver fibrosis in rats by detecting TGF-β1 gene and protein expressions. Methods A total of 48 SD rats were randomly divided into normal control, model control, glucyrrhizin aqueous,and total flavonoids groups,with 12 rats in each group. Except those in the normal control group, rats in other groups were intraperitoneally injected with 2 mL · kg-1 · d-1 carbon tetrachloride to induce liver fibrosis. Rats in glucyrrhizin aqueous group and total flavonoids ones were intragastrically administered with 15. 75 mg · kg-1 of glycyrrhizin aqueous solution or 35. 6 mg·kg-1 of total flavonoids aqueous solution,respectively. The normal and model control groups were administered with equal volume of aqueous solution. Thirty days later,rats were sacrificed by anesthesia. Livers were obtained to detect TGF-β1 gene and protein expressions by RT-PCR and Western-Blot. Results Relative gene expression of TGF-β1 in the normal control,model control,glucyrrhizin aqueous and flavonoids groups was 0. 725±0. 130,7. 493±1. 410,3. 016±1. 240,and 2. 668±1. 150,respectively. Total flavonoids from portulaca significantly reduced the gene (P<0. 01) and protein (P<0. 01) expressions of TGF-β1 . Conclusion Efficacy of total flavonoids from portulaca in treating hepatic fibrosis may be related to decreased TGF-β1 expression in rats.
