山东农业科学
山東農業科學
산동농업과학
SHANGDONG AGRICULTURAL SCIENCES
2014年
9期
17-20
,共4页
魏秀丽%高迎春%陈玲%徐恩民%章安源%张琦
魏秀麗%高迎春%陳玲%徐恩民%章安源%張琦
위수려%고영춘%진령%서은민%장안원%장기
整合子%基因盒%耐药性%大肠埃希菌%鸡源%山东
整閤子%基因盒%耐藥性%大腸埃希菌%鷄源%山東
정합자%기인합%내약성%대장애희균%계원%산동
Integron%Gene cassettes%Drug resistance%Escherichia coli%Chicken source%Shandong
研究了从山东省济南、德州等市肉鸡场分离的大肠埃希菌中Ⅰ类整合子及基因盒的流行状况。结果表明,整合酶基因检出率为53%;Ⅰ类整合子基因盒检出率为50%,大小为1085~2360 bp,100株细菌中各含1~2个基因盒,其中dfrA17+aadA5检出率最高。Ⅰ类整合子在多重耐药大肠埃希菌中广泛流行,是细菌多重耐药性产生和扩散的重要机制,养殖场应科学用药,加强耐药性监测。
研究瞭從山東省濟南、德州等市肉鷄場分離的大腸埃希菌中Ⅰ類整閤子及基因盒的流行狀況。結果錶明,整閤酶基因檢齣率為53%;Ⅰ類整閤子基因盒檢齣率為50%,大小為1085~2360 bp,100株細菌中各含1~2箇基因盒,其中dfrA17+aadA5檢齣率最高。Ⅰ類整閤子在多重耐藥大腸埃希菌中廣汎流行,是細菌多重耐藥性產生和擴散的重要機製,養殖場應科學用藥,加彊耐藥性鑑測。
연구료종산동성제남、덕주등시육계장분리적대장애희균중Ⅰ류정합자급기인합적류행상황。결과표명,정합매기인검출솔위53%;Ⅰ류정합자기인합검출솔위50%,대소위1085~2360 bp,100주세균중각함1~2개기인합,기중dfrA17+aadA5검출솔최고。Ⅰ류정합자재다중내약대장애희균중엄범류행,시세균다중내약성산생화확산적중요궤제,양식장응과학용약,가강내약성감측。
The epidemic situation of class Ⅰintegron and gene cassettes in Escherichia coli isolated from chicken farms of Jinan ,Dezhou,etc of Shandong Province were investigated .The results showed that the detec-tion rate of integrase gene was 53% and that of gene cassettes was 50%.The size of class Ⅰ integron gene cassettes ranged from 1 085 bp to 2 360 bp.Each of the 100 bacteria had 1 or 2 gene cassettes.Among which , the detection rate of dfrA17+aadA5 was the highest .This study showed that the class Ⅰintegron was widespread in multidrug resistant E.coli.And it was important molecular mechanism in the production and diffusion of multidrug resistance of bacteria .So, the farmers should use drugs scientifically and strengthen sur-veillance of antimicrobial resistance .