CAJ | 학술논문

目的探讨刺五加酸对急性肝损伤模型小鼠的保护作用。方法小鼠60只,随机分成6组,即正常对照组,模型对照组,阳性对照组(N-乙酰-L-半胱氨酸,NAC 300 mg·kg-1),刺五加酸小、中、大剂量(50,100,200 mg·kg-1)组,每组10只。连续给药3 d,末次给药前禁食16 h,给药1 h后除正常对照组外均灌胃给予他克林35 mg·kg-1。观察小鼠血清的生化指标和病理组织变化。结果刺五加酸大剂量组天冬氨酸氨基转移酶(143±46) U·L-1,丙氨酸氨基转移酶(32±9) U·L-1,乳酸脱氢酶(1218±312) U·L-1,丙二醛(3.24±0.48)μmol·g-1,谷胱甘肽(417±15) mg·g-1,均明显下降,与模型对照组比较均差异有统计学意义(P<0.05或P<0.01),肝损伤程度减轻。结论刺五加酸对急性肝损伤模型小鼠具有良好的保护作用。
목적탐토자오가산대급성간손상모형소서적보호작용。방법소서60지,수궤분성6조,즉정상대조조,모형대조조,양성대조조(N-을선-L-반광안산,NAC 300 mg·kg-1),자오가산소、중、대제량(50,100,200 mg·kg-1)조,매조10지。련속급약3 d,말차급약전금식16 h,급약1 h후제정상대조조외균관위급여타극림35 mg·kg-1。관찰소서혈청적생화지표화병리조직변화。결과자오가산대제량조천동안산안기전이매(143±46) U·L-1,병안산안기전이매(32±9) U·L-1,유산탈경매(1218±312) U·L-1,병이철(3.24±0.48)μmol·g-1,곡광감태(417±15) mg·g-1,균명현하강,여모형대조조비교균차이유통계학의의(P<0.05혹P<0.01),간손상정도감경。결론자오가산대급성간손상모형소서구유량호적보호작용。
Objective To study protective effects of acanthoic acid ( AA) against acute liver injury. Methods Sixty mice were randomly divided into six groups (n=10 each),including normal control group,model control group,positive control group (N-acetyl -L- cysteine,NAC,300 mg·kg-1),and AA at small (50 mg·kg-1),middle (100 mg·kg-1),and high (200 mg·kg-1 ) dose groups. Each group received respective treatment for 3 days and fasted for 16 h before the last dose. All animals except of the normal control group were treated with tacrine (35 mg·kg-1 ) 1 h after the treatments. Hepatic pathological and serum biochemical changes were observed. Results The high-dose of AA significantly reduced the levels of AST (143±46) U·L-1 ,ALT (32±9) U·L-1 ,LDH (1 218±312) U·L-1 ,MDA (3. 24±0. 48) μmol·g-1 ,and GSH (417±15) mg·g-1 compared with the model control group (P<0. 05 or P<0. 01). Liver injury was also ameliorated in AA high dose group.Conclusion AA has a protective effect on acute liver injury in mice.