CAJ | 학술논문

目的：肺癌的发生、发展与个体的遗传背景关系密切。文中旨在研究肺癌发病风险与短串联重复序列（ short tandem repeat ， STR）基因座的相关性，为深入探索肺癌的发生机制提供新的研究途径。方法采用PCR方法结合毛细管电泳对南京及其周边地区120例肺癌患者（病例组）和156例健康个体（对照组）的外周静脉血样进行15个STR基因座的多态性分析，根据2组在15个STR基因座的等位基因频率分布的差异性来推断与肺癌发生相关的易感因素或抗性因素。结果病例组与对照组分别在CSF1PO、D19S433、D3S1358基因座等位基因10、15．2、16之间的分布差异有统计学意义（P＜0．05）， OR值均＞1。病例组与对照组在CSF1PO、D19S433基因座等位基因14、14．2之间的分布差异有统计学意义（P＜0．05），OR值均＜1。结论 CSF1PO、D19S433、D3S1358基因座等位基因10、15．2、16可能是与肺癌发生相关的易感因素，CSF1PO、D19 S433基因座等位基因14、14．2是与肺癌发生相关的抗性因素。
목적：폐암적발생、발전여개체적유전배경관계밀절。문중지재연구폐암발병풍험여단천련중복서렬（ short tandem repeat ， STR）기인좌적상관성，위심입탐색폐암적발생궤제제공신적연구도경。방법채용PCR방법결합모세관전영대남경급기주변지구120례폐암환자（병례조）화156례건강개체（대조조）적외주정맥혈양진행15개STR기인좌적다태성분석，근거2조재15개STR기인좌적등위기인빈솔분포적차이성래추단여폐암발생상관적역감인소혹항성인소。결과병례조여대조조분별재CSF1PO、D19S433、D3S1358기인좌등위기인10、15．2、16지간적분포차이유통계학의의（P＜0．05）， OR치균＞1。병례조여대조조재CSF1PO、D19S433기인좌등위기인14、14．2지간적분포차이유통계학의의（P＜0．05），OR치균＜1。결론 CSF1PO、D19S433、D3S1358기인좌등위기인10、15．2、16가능시여폐암발생상관적역감인소，CSF1PO、D19 S433기인좌등위기인14、14．2시여폐암발생상관적항성인소。
Objective The onset and progression of lung cancer correlate closely to individual genetic background .This study evaluated the correlation between the onset risk of lung cancer and short tandem repeat (STR) polymorphisms in order to find a new pathway for investigating the pathogenesis of lung cancer . Methods Using PCR and electrophoresis on 15 STR loci , we studied the gene and genotype frequencies of peripheral vein blood specimens from 120 lung cancer patients and 156 healthy individuals in Nan-jing area.According to the differences in the allele distribution of the 15 STR loci, we evaluated the susceptive and/or resistant factors relevant to lung cancer . Results There were statistically significant differences between the lung cancer patients and healthy controls at alleles 10, 15.2, and 16 of the loci CSF1PO, D19S433, and D3S1358 (P<0.05, OR>1), as well as at alleles 14 and 14.2 of the loci CSF1PO and D19S433 (P<0.05, OR<1). Conclusion It is possible that alleles 10, 15.2 and 16 of the loci CSF1PO, D19S433, and D3S1358 are susceptive factors and alleles 14 and 14.2 of the loci CSF1PO and D19S433 are resistant factors relevant to lung cancer .