CAJ | 학술논문

目的：肿瘤高表达多药耐药相关转运蛋白三磷酸腺苷结合转运蛋白 G家族成员2（ ATP-binding cassette super fam-ily G member 2， ABCG2）是造成耐药及化疗失败的主要原因。文中旨在探讨环磷酰胺体内干预后肾上腺皮质癌细胞中ABCG2表达的意义。方法 BALB/C-nu裸鼠共10只，雌雄不拘，随机数表法分为对照组和环磷酰胺组，每组5只。将SW-13细胞注射入裸鼠皮下建立裸鼠皮下移植瘤模型，环磷酰胺组予腹腔注射环磷酰胺，对照组予注射等量等渗盐水，并对获得的2组瘤体组织进行原代培养。用免疫组化法（阳性肿瘤细胞百分比＜10％者为阴性，≥10％为阳性）和流式细胞法分别检测瘤体组织及原代细胞株ABCG2的表达。结果流式细胞法检测ABCG2在环磷酰胺组细胞中表达率[（97．89±1．36）％]高于对照组细胞[（81．88±8．31）％]，差异有统计学意义（P＜0．05），免疫组化结果提示环磷酰胺组瘤体组织中ABCG2表达阳性率[（69．1±1．83）％]高于对照组[（53．4±1．65）％]，差异有统计学意义（P＜0．05）。结论多药耐药基因ABCG2参与肾上腺皮质癌耐药，可能为肾上腺皮质癌干预靶点。
목적：종류고표체다약내약상관전운단백삼린산선감결합전운단백 G가족성원2（ ATP-binding cassette super fam-ily G member 2， ABCG2）시조성내약급화료실패적주요원인。문중지재탐토배린선알체내간예후신상선피질암세포중ABCG2표체적의의。방법 BALB/C-nu라서공10지，자웅불구，수궤수표법분위대조조화배린선알조，매조5지。장SW-13세포주사입라서피하건립라서피하이식류모형，배린선알조여복강주사배린선알，대조조여주사등량등삼염수，병대획득적2조류체조직진행원대배양。용면역조화법（양성종류세포백분비＜10％자위음성，≥10％위양성）화류식세포법분별검측류체조직급원대세포주ABCG2적표체。결과류식세포법검측ABCG2재배린선알조세포중표체솔[（97．89±1．36）％]고우대조조세포[（81．88±8．31）％]，차이유통계학의의（P＜0．05），면역조화결과제시배린선알조류체조직중ABCG2표체양성솔[（69．1±1．83）％]고우대조조[（53．4±1．65）％]，차이유통계학의의（P＜0．05）。결론다약내약기인ABCG2삼여신상선피질암내약，가능위신상선피질암간예파점。
Objective High expression of multi-resistant transporter ATP-binding cassette super family G member 2 (ABCG2) is a major cause of drug resistance and chemotherapeutic failure of cancer .This study was to investigate the significance of ABCG2 expression in adrenocortical cancer cells after cyclophosphamide ( CTX) intervention in vivo . Methods Ten male and fe-male BALB/C-nu mice were randomly divided into a cyclophosphamide ( CTX) group and a control of equal number .SW-13 cells were subcutaneously injected into the nude mice to establish a model of subcutaneous transplantation tumor , followed by intraperitoneal injec-tion of CTX and isotonic saline solution into the two groups of mice , respectively .Then the expression of ABCG 2 in tumor tissue and primarily cultured cells was detected by immunohistochemistry and flow cytometry . Results The expression of ABCG 2 in the tumor tissue was significantly higher in the CTX than in the control group ([69.1 ±1.83]%vs [53.4 ±1.65]%, P<0.05), and so was that in the primarily cultured cells ([97.89 ±1.36]% vs [81.88 ±8.31]%, P<0.05). Conclusion The ABCG2 gene is in-volved in the drug resistance of adrenocortical carcinoma and may be a therapeutic target of the malignancy .