CAJ | 학술논문

为了解垫料基质中细菌的群落结构多样性，应用PCR-DGGE技术对发酵床7种不同垫料（锯木屑、稻壳、酒糟、菌糠、醋糟、稻草、稻壳炭）的细菌群落结构进行了研究，根据DGGE指纹图谱，对它们的细菌群落多样性和优势条带进行了分析。结果表明，垫料样品的细菌多样性指数、丰富度均有所不同，酒糟垫料组细菌多样性指数最高，稻草垫料组细菌多样性指数最低。全锯木屑与50%稻壳相似性较高而聚为一类，与50%菌糠次之，与50%稻草的相似性最低。在垫料基质中检测到的菌群主要是节杆菌属（Arthrobacter sp.）、Amaricoccu sp.、马杜拉放线菌属（Actinomadura sp.）、芽孢杆菌属（Bacillales sp.）、梭菌属（Clostridium sp.）、肠杆菌属（Escherichia sp.）、细杆菌属（Microbacterium sp.）、假单胞菌属（Pseudomonas sp.）、红球菌属（Rhodococcus sp.）、葡萄球菌属（Staphylococcus sp.），以及一些未知的菌群。垫料组成是影响发酵床垫料微生物构成的重要因素，稻壳、菌糠作为垫料可部分替代锯木屑，而对发酵床垫料的微生物区系影响较小。
위료해점료기질중세균적군락결구다양성，응용PCR-DGGE기술대발효상7충불동점료（거목설、도각、주조、균강、작조、도초、도각탄）적세균군락결구진행료연구，근거DGGE지문도보，대타문적세균군락다양성화우세조대진행료분석。결과표명，점료양품적세균다양성지수、봉부도균유소불동，주조점료조세균다양성지수최고，도초점료조세균다양성지수최저。전거목설여50%도각상사성교고이취위일류，여50%균강차지，여50%도초적상사성최저。재점료기질중검측도적균군주요시절간균속（Arthrobacter sp.）、Amaricoccu sp.、마두랍방선균속（Actinomadura sp.）、아포간균속（Bacillales sp.）、사균속（Clostridium sp.）、장간균속（Escherichia sp.）、세간균속（Microbacterium sp.）、가단포균속（Pseudomonas sp.）、홍구균속（Rhodococcus sp.）、포도구균속（Staphylococcus sp.），이급일사미지적균군。점료조성시영향발효상점료미생물구성적중요인소，도각、균강작위점료가부분체대거목설，이대발효상점료적미생물구계영향교소。
Previous research has shown that different digestion efficiency of various bedding litters in pig biobeds is linked to bacterial vari-ation. Here we examined both bacterial community structure and predominant bands in biobeds with seven different bedding litters(full saw-dust, rice husk, distiller’s grains, mushroom residue, vinegar residue, rice straw and rice husk charcoal)using PCR-DGGE technique. The Shannon-Wiener index and richness had significant differences in different litters, with the highest Shannon-Wiener index found in the dis-tiller’s grains but the lowest in the rice straw. Bacterial community structure in 50%rice husk showed high similarity with that in full saw-dust, followed by 50% mushroom residue, while the lowest similarity found in 50% straw. Bacterial strains detected in bedding litters be-longed to Arthrobacter sp., Amaricoccu sp., Actinomadura sp., Bacillales sp., Clostridium sp., Escherichia sp., Microbacterium sp., Pseu-domonas sp., Rhodococcus sp., Staphylococcus sp. and some unclassified-bacteria. In conclusion, bedding litter composition is an important factor influencing bacterial community structure of biobeds. Rice husk and mushroom residues could partially replace sawdust as bedding materials with little impacts on the microbial community.