农业环境科学学报
農業環境科學學報
농업배경과학학보
Journal of Agro-Environment Science
2014年
9期
1736-1742
,共7页
贺根和%王小东%刘强%吴吉春
賀根和%王小東%劉彊%吳吉春
하근화%왕소동%류강%오길춘
铝胁迫%酸性红壤%真菌种群多样性%PCR-RFLP
鋁脅迫%痠性紅壤%真菌種群多樣性%PCR-RFLP
려협박%산성홍양%진균충군다양성%PCR-RFLP
aluminum stress%acidic red soils%fungal community diversity%PCR-RFLP
通过聚合酶链式反应-限制性片段长度多态(PCR-RFLP)技术构建了红壤真菌的内转录间隔区(Internal Transcribed Spacer, ITS)rDNAs文库,比较分析了红壤地区典型森林土和农田土真菌群落结构对铝胁迫的响应,构建了6个土壤样品的真菌ITS rDNAs文库。从这6个克隆文库中随机挑取克隆进行了PCR-RFLP指纹图谱分析,共获得77个独特的真菌操作分类单元(Operational Taxanomical Uints, OTUs)。对77个OTUs的代表性克隆测序并利用Blast工具进行分析(相似性95%~100%)。研究结果表明森林土壤和农田土壤样品中真菌类群79.2%(61)属于子囊菌门(Ascomycota)、担子菌门(Basidiomycota)和球囊菌门(Glomeromycota),20.8%(16)属于未分类的簇(Unclassified fungi),其中担子菌门真菌在两种土壤中具有明显优势。物种多样性指数(Simpson、Shannon和Chao1)分析表明,铝胁迫降低了土壤真菌种群的多样性,农田土壤中真菌的种群多样性明显高于森林土壤。而且随着铝胁迫浓度的增加,担子菌门真菌在两种土壤中丰度明显下降,子囊菌门真菌的丰度显著上升,在高铝处理浓度处理的土壤中演变为优势种群。
通過聚閤酶鏈式反應-限製性片段長度多態(PCR-RFLP)技術構建瞭紅壤真菌的內轉錄間隔區(Internal Transcribed Spacer, ITS)rDNAs文庫,比較分析瞭紅壤地區典型森林土和農田土真菌群落結構對鋁脅迫的響應,構建瞭6箇土壤樣品的真菌ITS rDNAs文庫。從這6箇剋隆文庫中隨機挑取剋隆進行瞭PCR-RFLP指紋圖譜分析,共穫得77箇獨特的真菌操作分類單元(Operational Taxanomical Uints, OTUs)。對77箇OTUs的代錶性剋隆測序併利用Blast工具進行分析(相似性95%~100%)。研究結果錶明森林土壤和農田土壤樣品中真菌類群79.2%(61)屬于子囊菌門(Ascomycota)、擔子菌門(Basidiomycota)和毬囊菌門(Glomeromycota),20.8%(16)屬于未分類的簇(Unclassified fungi),其中擔子菌門真菌在兩種土壤中具有明顯優勢。物種多樣性指數(Simpson、Shannon和Chao1)分析錶明,鋁脅迫降低瞭土壤真菌種群的多樣性,農田土壤中真菌的種群多樣性明顯高于森林土壤。而且隨著鋁脅迫濃度的增加,擔子菌門真菌在兩種土壤中豐度明顯下降,子囊菌門真菌的豐度顯著上升,在高鋁處理濃度處理的土壤中縯變為優勢種群。
통과취합매련식반응-한제성편단장도다태(PCR-RFLP)기술구건료홍양진균적내전록간격구(Internal Transcribed Spacer, ITS)rDNAs문고,비교분석료홍양지구전형삼림토화농전토진균군락결구대려협박적향응,구건료6개토양양품적진균ITS rDNAs문고。종저6개극륭문고중수궤도취극륭진행료PCR-RFLP지문도보분석,공획득77개독특적진균조작분류단원(Operational Taxanomical Uints, OTUs)。대77개OTUs적대표성극륭측서병이용Blast공구진행분석(상사성95%~100%)。연구결과표명삼림토양화농전토양양품중진균류군79.2%(61)속우자낭균문(Ascomycota)、담자균문(Basidiomycota)화구낭균문(Glomeromycota),20.8%(16)속우미분류적족(Unclassified fungi),기중담자균문진균재량충토양중구유명현우세。물충다양성지수(Simpson、Shannon화Chao1)분석표명,려협박강저료토양진균충군적다양성,농전토양중진균적충군다양성명현고우삼림토양。이차수착려협박농도적증가,담자균문진균재량충토양중봉도명현하강,자낭균문진균적봉도현저상승,재고려처리농도처리적토양중연변위우세충군。
Aluminum(Al)in acidic soils shows toxicity to soil microbes. In this study, we constructed fungal internal transcribed spacer (ITS)rDNAs clone library using the PCR- amplified restriction fragment length polymorphism(PCR-RFLP)method, and comparatively studied the responses of fungal community in forest soil and agriculture soil to Al stress. A total of six fungal clone communities was obtained from the tested soil samples. Clones of each library were randomly selected for PCR-RFLP analysis of ITS rDNA fragments. Totally 77 genotypes were identified by RFLP fingerprinting and sequenced. These 77 genotypes were then sequenced and their respective phylotypes were identified through the Basic Local Alignment Search Tool(BLAST)of the National Center for Biotechnology Information(NCBI)with similarity of 95%~100%. Sequence results revealed that 79.2%(61)of the clone sequences have the highest similarity with those of the A s-comycota, Basidiomycota and Glomeromycota, and 20.8%(16)have near similarity with unclassified fungi. Basidiomycota was predominant in both soils. The Simpson, Shannon and Chao1 indices all showed that Al addition reduced the richness and diversity of soil fungi. Howev-er, the diversity of fungal communities was lower in the forest soil than in the agriculture soil. Elevated Al increased the relative abundance of Ascomycota but decreased that of Basidiomycota, thus evolving into dominant population in high Al soils.