CAJ | 학술논문

通过盆栽试验比较分析黄瓜发育早期叶片亚显微结构对不同浓度（0、6、10、20、40 mg·kg-1）DEHP胁迫的响应。透射电镜观察发现，DEHP胁迫对黄瓜叶肉细胞亚显微结构造成了不同程度损伤，随着DEHP处理浓度的增大，液泡逐渐膨大，细胞的膜结构消失。当DEHP达到10 mg·kg-1时，线粒体嵴数目减少，线粒体呈空泡状；同时叶绿体类囊体膨胀，直至破裂，叶绿体被膜受到破坏，叶绿体中淀粉粒明显膨大、增多；当DEHP达到20 mg·kg-1时，细胞核核仁解体，染色质凝聚，核膜断裂。与亚显微观察结果相一致，随着DEHP浓度的增加，黄瓜叶片中的叶绿素a、b和总叶绿素含量均呈现下降的趋势，而过氧化氢（H2O2）含量则呈上升趋势，表明DEHP胁迫通过产生过量的活性氧破坏黄瓜叶片正常的亚显微结构。
통과분재시험비교분석황과발육조기협편아현미결구대불동농도（0、6、10、20、40 mg·kg-1）DEHP협박적향응。투사전경관찰발현，DEHP협박대황과협육세포아현미결구조성료불동정도손상，수착DEHP처리농도적증대，액포축점팽대，세포적막결구소실。당DEHP체도10 mg·kg-1시，선립체척수목감소，선립체정공포상；동시협록체류낭체팽창，직지파렬，협록체피막수도파배，협록체중정분립명현팽대、증다；당DEHP체도20 mg·kg-1시，세포핵핵인해체，염색질응취，핵막단렬。여아현미관찰결과상일치，수착DEHP농도적증가，황과협편중적협록소a、b화총협록소함량균정현하강적추세，이과양화경（H2O2）함량칙정상승추세，표명DEHP협박통과산생과량적활성양파배황과협편정상적아현미결구。
Due to its wide uses, di- 2-ethylhexyl phthalate(DEHP)is everywhere in the environment. It has been detected in most agri-cultural soils in China. It may affect the growth and development of plants. A comparative analysis of the influence of different concentra-tions of DEHP(0, 6, 10, 20, 40 mg·kg-1)on ultrastructure of cucumber seedling leaves at the early growth stages was performed in a pot ex-periment. Transmission electron microscope(TEM)was employed to examine the effects of DEHP on the ultrastructure of the leaves. In-creasing DEHP concentrations led to expanded vacuoles and collapsed structure of cell membrane. At 10 mg·kg-1 of DEHP, mitochondria became vacuolized, and the number of mitochondrial cristae decreased. Meanwhile, thylakoids of chloroplast swelled gradually, chloroplast envelopes were destroyed, and the quantity and volume of starch grains in the chloroplast increased. At 20 mg·kg-1 DEHP, the nucleolus be-came integrated, the chromatin of nucleus was agglutinated, and the nuclear membrane was disrupted. Consistent with the ultrastructure re-sults, the contents of chlorophyll a, chlorophyll b and total chlorophyll decreased while the content of hydrogen peroxide(H2O2)increased with increasing concentrations of DEHP, indicating that DEHP stress caused damages to the ultrastructure of cucumber leaves by overpro-ducing reactive oxygen species.