CAJ | 학술논문

目的：探讨甘氨双唑钠对放射线照射后喉癌细胞AT突变基因( AT mutated, ATM)表达的影响。方法将喉癌Hep-2细胞分为空白组(不做任何处理)、照射组(接受5 Gy X线照射)、观察组(甘氨双唑钠+照射组),经浓度为0.8 mmol/L甘氨双唑钠100μl作用4 h后,再接受5 Gy X线照射,分别采用逆转录聚合酶链反应(RT-PCR)和蛋白免疫印迹杂交技术检测ATM mRNA及蛋白表达量。结果空白组、照射组、观察组ATM mRNA相对表达量分别为0.727±0.024、0.955±0.046、0.272±0.038,3组间两两比较差异均有统计学意义(P<0.05)；经甘氨双唑钠干预后喉癌Hep-2细胞ATM蛋白表达水平明显下降,3组间两两比较差异亦均有统计学意义( P<0.05)。结论甘氨双唑钠可能通过下调ATM的表达实现对喉癌细胞的放射增敏效应。
목적：탐토감안쌍서납대방사선조사후후암세포AT돌변기인( AT mutated, ATM)표체적영향。방법장후암Hep-2세포분위공백조(불주임하처리)、조사조(접수5 Gy X선조사)、관찰조(감안쌍서납+조사조),경농도위0.8 mmol/L감안쌍서납100μl작용4 h후,재접수5 Gy X선조사,분별채용역전록취합매련반응(RT-PCR)화단백면역인적잡교기술검측ATM mRNA급단백표체량。결과공백조、조사조、관찰조ATM mRNA상대표체량분별위0.727±0.024、0.955±0.046、0.272±0.038,3조간량량비교차이균유통계학의의(P<0.05)；경감안쌍서납간예후후암Hep-2세포ATM단백표체수평명현하강,3조간량량비교차이역균유통계학의의( P<0.05)。결론감안쌍서납가능통과하조ATM적표체실현대후암세포적방사증민효응。
Objective To evaluate the expression of ataxia-telangiectasia mutated (ATM) gene in laryngeal cancer cells after the intervention of combined sodium glycididazole and radiation. Methods Laryngeal cancer Hep-2 cells were di-vided into the normal group, the irradiation group (5 Gy of X-radiation) and the observation group (irradiation plus sodium glycididazole group) . In observation group, laryngeal cancer cells received 5 Gy of X-radiation at 4 h after the intervention of 100 μl of sodium glycididazole (concentration of 0. 8 mmol/L). The ATM mRNA and protein expression of laryngeal cancer cells were detected by RT-PCR and Western blot hybridization, respectively. Results The ATM mRNA expression levels in normal group, irradiation group and observation group were 0. 727±0. 024, 0. 955±0. 046 and 0. 272±0. 038, respectively. The ATM mRNA and protein expression of laryngeal cancer Hep-2 cells declined after the intervention of sodium glycididazole. The differences of ATM mRNA and protein expression levels among the three groups were significant (P<0. 05). Conclusion Sodium glycididazole could enhance radiosensitivity of laryngeal cancer cells through down-regulation of the ATM expres-sion.