北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2014年
10期
70-71
,共2页
玉米浆%多粘菌素E%发酵%正交试验
玉米漿%多粘菌素E%髮酵%正交試驗
옥미장%다점균소E%발효%정교시험
Corn steep liquor%Polymyxin E%Fermentation%Orthogonal experiments
目的:试验比较及评价不同组分玉米浆对多粘菌素E发酵生产的影响。方法:以多粘芽孢杆菌为对象,通过不同产地玉米浆对比试验和四因素三水平正交试验确定多粘菌素E发酵配方中玉米浆原材料最佳组分及配比。结果:该菌种使用的原材料玉米浆最佳组分及配比条件:胱氨酸1700mg/100g,异亮氨酸1050mg/100g,丝氨酸950mg/100g,组氨酸1300mg/100g。结论:玉米浆质量的波动是引起多粘菌素E发酵单位波动的重要原因之一,要保持稳定的发酵水平就必须对不同产地生产的玉米浆进行摇瓶试验,以确定最合适的玉米浆用于生产。
目的:試驗比較及評價不同組分玉米漿對多粘菌素E髮酵生產的影響。方法:以多粘芽孢桿菌為對象,通過不同產地玉米漿對比試驗和四因素三水平正交試驗確定多粘菌素E髮酵配方中玉米漿原材料最佳組分及配比。結果:該菌種使用的原材料玉米漿最佳組分及配比條件:胱氨痠1700mg/100g,異亮氨痠1050mg/100g,絲氨痠950mg/100g,組氨痠1300mg/100g。結論:玉米漿質量的波動是引起多粘菌素E髮酵單位波動的重要原因之一,要保持穩定的髮酵水平就必鬚對不同產地生產的玉米漿進行搖瓶試驗,以確定最閤適的玉米漿用于生產。
목적:시험비교급평개불동조분옥미장대다점균소E발효생산적영향。방법:이다점아포간균위대상,통과불동산지옥미장대비시험화사인소삼수평정교시험학정다점균소E발효배방중옥미장원재료최가조분급배비。결과:해균충사용적원재료옥미장최가조분급배비조건:광안산1700mg/100g,이량안산1050mg/100g,사안산950mg/100g,조안산1300mg/100g。결론:옥미장질량적파동시인기다점균소E발효단위파동적중요원인지일,요보지은정적발효수평취필수대불동산지생산적옥미장진행요병시험,이학정최합괄적옥미장용우생산。
Objective: To test comparison and evaluation of different components of corn steep liquor of polymyxin E fermentation production.Methods: more sticky bacillus as object, through different producers of corn steep liquor contrast test four factors and three levels orthogonal test to determine the polymyxin E fermented corn steep liquor raw materials ratio of components and the best formula. Results:the best components of the use of the raw materials of strains of corn steep liquor and the matching condition:cystine 1700mg/100g, isoleucine 1050mg/100g, isoleucine serine 950mg/100g, histidine 1300mg/100g. Conclusion:corn steep liquor quality of fluctuations is causing polymyxin E fermentation units, one of the important reasons to maintain the stability of the fermentation level of different origin production of corn steep liquor must shake flask test, to determine the optimum corn steep liquor used in the production.