CAJ | 학술논문

目的：检测Syn、Bax、Bcl-2蛋白在帕金森病大鼠模型结肠中的表达及意义，探讨帕金森病消化系统功能紊乱的可能机制。方法采用羟多巴胺（6-hydroxydopamine ，6-O HDA ）损毁大鼠中枢黑质多巴胺能神经元，诱导制备实验性帕金森病大鼠模型。应用免疫组化法S-P法检测Syn、Bax、Bcl-2在帕金森病模型大鼠及正常对照组大鼠结肠组织中的表达情况。结果造模成功后，用免疫组化法S-P法，发现帕金森模型大鼠结肠中的Syn和 Bax表达水平较对照组增加，而Bcl-2表达水平较对照组减少。结论帕金森大鼠结肠组织中的Syn及Bax表达增加，Bcl-2表达减少，为研究帕金森患者肠道功能紊乱的机制提供线索。
목적：검측Syn、Bax、Bcl-2단백재파금삼병대서모형결장중적표체급의의，탐토파금삼병소화계통공능문란적가능궤제。방법채용간다파알（6-hydroxydopamine ，6-O HDA ）손훼대서중추흑질다파알능신경원，유도제비실험성파금삼병대서모형。응용면역조화법S-P법검측Syn、Bax、Bcl-2재파금삼병모형대서급정상대조조대서결장조직중적표체정황。결과조모성공후，용면역조화법S-P법，발현파금삼모형대서결장중적Syn화 Bax표체수평교대조조증가，이Bcl-2표체수평교대조조감소。결론파금삼대서결장조직중적Syn급Bax표체증가，Bcl-2표체감소，위연구파금삼환자장도공능문란적궤제제공선색。
Objective To detect the expression and significance of Syn ,Bax ,Bcl-2 in colon of rat model of Parkinson's dis-ease ,and to explore the possible mechanism of digestive system disorder of Parkinson's disease. Methods 6-hydroxydopamine (6-OHDA) damaged rat central dopaminergic neurons in the substantia nigra were used to prepare experimental rat model of Parkinson's disease.S-P method of immunohistochemical was applied to detect the expression of Syn ,Bax ,Bcl-2 of rats'colon tissue in rat model of Parkinson's disease and normal control group.Results After the success of the model ,compared with the control group ,Syn and Bax expression levels increased in colon of rat model of Parkinson's disease by immunohistochemical S-P method ,while the Bcl-2 expression level was reduced.Conclusion The expressions of Syn and Bax in colon tissue of rat model of Parkinson's disease increase ,but the expression of Bcl-2 reduces ,which provide clues to research the mechanism of intestinal dysfunction in Parkinson's disease.