中国医药指南
中國醫藥指南
중국의약지남
CHINA MEDICINE GUIDE
2014年
25期
6-6,8
,共2页
唐何%毛谊平%林海%李子君%任荣军
唐何%毛誼平%林海%李子君%任榮軍
당하%모의평%림해%리자군%임영군
高效液相色谱%骨伤愈合胶囊%三七皂苷R1
高效液相色譜%骨傷愈閤膠囊%三七皂苷R1
고효액상색보%골상유합효낭%삼칠조감R1
HPLC%Gushang Yuhe Capsules%Notoginsenoside R1
目的：建立HPLC法测定骨伤愈合胶囊中三七皂苷R1含量。方法色谱柱为Agilent ZORBAX SB-C18（250 mm×4.6 mm，5μm）；流动相为乙腈-0.4%磷酸溶液梯度洗脱，流速为1.0 mL/min，检测波长为203 nm，柱温为30℃。结果三七皂苷R1在0.2048~2.0480μg范围内线性关系良好，r=0.9999（n=5），平均回收率为99.0%（n=9），RSD为0.8%。结论该方法灵敏、准确、专属性强，能简便有效控制骨伤愈合胶囊中三七皂苷R1的含量。
目的：建立HPLC法測定骨傷愈閤膠囊中三七皂苷R1含量。方法色譜柱為Agilent ZORBAX SB-C18（250 mm×4.6 mm，5μm）；流動相為乙腈-0.4%燐痠溶液梯度洗脫，流速為1.0 mL/min，檢測波長為203 nm，柱溫為30℃。結果三七皂苷R1在0.2048~2.0480μg範圍內線性關繫良好，r=0.9999（n=5），平均迴收率為99.0%（n=9），RSD為0.8%。結論該方法靈敏、準確、專屬性彊，能簡便有效控製骨傷愈閤膠囊中三七皂苷R1的含量。
목적：건립HPLC법측정골상유합효낭중삼칠조감R1함량。방법색보주위Agilent ZORBAX SB-C18（250 mm×4.6 mm，5μm）；류동상위을정-0.4%린산용액제도세탈，류속위1.0 mL/min，검측파장위203 nm，주온위30℃。결과삼칠조감R1재0.2048~2.0480μg범위내선성관계량호，r=0.9999（n=5），평균회수솔위99.0%（n=9），RSD위0.8%。결론해방법령민、준학、전속성강，능간편유효공제골상유합효낭중삼칠조감R1적함량。
Objective To establish an HPLC method for determination the content of Notoginsenoside R1 in Gushang Yuhe Capsules.Methods The column was Agilent ZORBAX SB-C18(250 mm×4.6 mm,5 μm)and the temperature was 30℃;The mobile phase was acetonitrile-0.4% phosphoric acid solution with linear gradient elution, detection was done at 203nm and a flow rate of 1.0 mL/min.Results Notoginsenoside R1 has a good linear relationships in 0.2048~2.048 μg(r=0.9999);the average recovery was 98.97%(n=9,RSD was 0.8%.Conclusion The methods are sensitive, accurate and specific,which can be used for the quality control of Gushang Yuhe Capsules.
