CAJ | 학술논문

目的：探讨脂联素对RAW264.7巨噬细胞源性泡沫细胞ABCA1及胆固醇含量的影响及其可能的机制。方法体外培养RAW264.7细胞，加入20 mg/L氧化低密度脂蛋白（ox-LDL）共同孵育48 h，将其诱导成泡沫细胞，加入不同浓度（0、1、5、10μg/mL）的脂联素干预24 h，RT-PCR测定ABCA1 mRNA的表达，高效液相色谱测定细胞内胆固醇含量。观察脂联素对泡沫细胞中ABCA1表达的影响。结果脂联素显著增加RAW264.7巨噬细胞源性泡沫细胞ABCA1 mRNA的表达（P＜0.05），并增加细胞内胆固醇含量，且呈浓度依赖性（P＜0.05）。结论脂联素可以增加巨噬源性泡沫细胞ABCA1转录水平，促进胆固醇流出，延缓AS的发生发展。
목적：탐토지련소대RAW264.7거서세포원성포말세포ABCA1급담고순함량적영향급기가능적궤제。방법체외배양RAW264.7세포，가입20 mg/L양화저밀도지단백（ox-LDL）공동부육48 h，장기유도성포말세포，가입불동농도（0、1、5、10μg/mL）적지련소간예24 h，RT-PCR측정ABCA1 mRNA적표체，고효액상색보측정세포내담고순함량。관찰지련소대포말세포중ABCA1표체적영향。결과지련소현저증가RAW264.7거서세포원성포말세포ABCA1 mRNA적표체（P＜0.05），병증가세포내담고순함량，차정농도의뢰성（P＜0.05）。결론지련소가이증가거서원성포말세포ABCA1전록수평，촉진담고순류출，연완AS적발생발전。
Objective Effects of adiponectin on RAW264.7 macrophage derived foam cells and cholesterol content to ABCA1) objective and its possible mechanisms.Methods RAW264.7 cells cultured in vitro, joined the 20mg/L oxidized low density lipoprotein(ox-LDL) were incubated for 48 h, the induced into foam cells, with different concentrations(0,1,5,10 μg/mL) of adiponectin 24h expression of RT-PCR ABCA1 mRNA, determination, HPLC determination of intracellular cholesterol content. To observe the effects of adiponectin on the expression of ABCA1 in foam cells.Results The expression of adiponectin was significantly increased in RAW264.7 macrophage derived foam cells in ABCA1 mRNA(P<0.05), and increased cellular cholesterol content, in a dose-dependent manner(P<0.05).Conclusion Adiponectin can increase the macrophage derived foam cell ABCA1 transcription level, promote cholesterol efflux, delay the occurrence and development of AS.