CAJ | 학술논문

目的：探讨凋亡抑制蛋白（ Survivin）对食管癌ECA-l09细胞株的生物学影响。方法通过脂质体介导将Survivin基因siRNA 瞬时转染ECA-l09细胞。采用RT-PCR及Western blot 检测转染后ECA-l09细胞内Survivin 基因的表达水平。MTT比色法、流式细胞术（ FCM）及Western blot观察细胞增殖、凋亡、周期及Caspase-9蛋白表达变化情况。结果 RT-PCR及Western blot证实，siRNA沉默的ECA-l09细胞中Survivin mRNA、蛋白的表达水平较未转染组明显降低（ P<0.05）。MTT、细胞凋亡及周期结果表明，siRNA沉默的ECA-l09细胞其增殖能力明显减弱、细胞凋亡明显升高，G2/M期阻滞，Caspase-9蛋白表达增加（ P<0.05）。结论 Survivin参与食管癌细胞增殖、凋亡、周期这一生物学过程。
목적：탐토조망억제단백（ Survivin）대식관암ECA-l09세포주적생물학영향。방법통과지질체개도장Survivin기인siRNA 순시전염ECA-l09세포。채용RT-PCR급Western blot 검측전염후ECA-l09세포내Survivin 기인적표체수평。MTT비색법、류식세포술（ FCM）급Western blot관찰세포증식、조망、주기급Caspase-9단백표체변화정황。결과 RT-PCR급Western blot증실，siRNA침묵적ECA-l09세포중Survivin mRNA、단백적표체수평교미전염조명현강저（ P<0.05）。MTT、세포조망급주기결과표명，siRNA침묵적ECA-l09세포기증식능력명현감약、세포조망명현승고，G2/M기조체，Caspase-9단백표체증가（ P<0.05）。결론 Survivin삼여식관암세포증식、조망、주기저일생물학과정。
Objective To investiGate the bioloGical effect of inhibitor of apoptosis proteins( Survivin) on esophaGeal cancer ECA-l09 cell line. Methods Survivin siRNA was transiently transfected into non small esophaGeal cancer ECA-l09 cell line by liposome-mediated method and the expression level of Survivin siRNA was detected by RT-PCR and western blot. MTT assay,cell apoptosis,cell cycle and western blot were conducted as to observe proliferation,apoptosis,cycle of cell and expression of Caspase-9. Results RT-PCR and Western blot showed that ECA-l09 cell transfected Survivin siRNA had a lower relative expressive content compared with untransfected Group( P <0. 05 ). MTT assay,cell apoptosis and cell cycles demonstrated that ECA-l09 cell transfected Survivin siRNA had a lower survival fraction,hiGher cell apoptosis,more percentaGe of the G2/M phases( P < 0. 05 ). Conclusion Survivin involve in the bioloGical processes of esophaGeal cancer cell proliferation,apoptosis,and cell cycle.