CAJ | 학술논문

在已获得水稻泛素连接酶基因Os H UB2过表达转基因植株的基础上，根据 Os H UB2基因序列设计3对引物，分别为 OsHUB2-1、OsHUB2-2、OsHUB2-3，通过 RT-PCR筛选条带特异单一的引物， real time PCR （qRT-PCR）方法验证最适合检测OsHUB2的反应条件。结果表明OsHUB2-F3R3扩增的PCR产物特异性最强，扩增曲线及熔解曲线等指标都在理想范围内，同时该引物能很好地区分对照及转基因材料，进而筛选出Os H UB2基因上调表达的转基因植株。Realtime体系的建立与优化为今后进一步研究水稻泛素连接酶基因Os H UB2的功能奠定了基础。
재이획득수도범소련접매기인Os H UB2과표체전기인식주적기출상，근거 Os H UB2기인서렬설계3대인물，분별위 OsHUB2-1、OsHUB2-2、OsHUB2-3，통과 RT-PCR사선조대특이단일적인물， real time PCR （qRT-PCR）방법험증최괄합검측OsHUB2적반응조건。결과표명OsHUB2-F3R3확증적PCR산물특이성최강，확증곡선급용해곡선등지표도재이상범위내，동시해인물능흔호지구분대조급전기인재료，진이사선출Os H UB2기인상조표체적전기인식주。Realtime체계적건립여우화위금후진일보연구수도범소련접매기인Os H UB2적공능전정료기출。
(1 .RiceResearch Institute ,FujianAcademy of Agricultural Sciences ,Fuzhou,Fujian 350019 ,China;2 . Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China ,Ministry of Agriculture ,P.R China/Fuzhou branch ,National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding ,Fuzhou , Fujian 350003 ,China;3 . Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology ,P.R.China , Fuzhou ,Fujian 350003 ,China;4 . National Rice Engineering Laboratory of China ,Fuzhou , Fujian 350003 ,China;5 . South China Bases of National Key Laboratory of Hybrid Rice for China ,Fuzhou ,Fujian 350003 ,China;6.Fruit Research Institute ,Fujian Academy of Agricultural Sciences ,Fuzhou ,Fujian 350013 ,China)and OsHUB2-F3R3 ,were designed using the Primer Premier 5.0 according to the sequence of the enzyme from GeneBank .Subsequently ,RT-PCR was conducted to select the best primer pairs with a specific and single amplification product . The results showed OsHUB2-F3R3 to be desirable on both and functions . The analytical procedure was thus considered of use in future studies on the rice ubiquitin ligase .