甘肃农业大学学报
甘肅農業大學學報
감숙농업대학학보
JOURNAL OF GANSU AGRICULTURAL UNIVERSITY
2014年
4期
136-142
,共7页
大花宣草%再生体系%外源激素
大花宣草%再生體繫%外源激素
대화선초%재생체계%외원격소
Hemerocallis middendorfii Trautv.et Mey%regeneration system%hormonone
以萱草‘香宝’的花托为外植体,用正交试验法研究了不同激素及其不同浓度对愈伤组织诱导、愈伤组织增殖、不定芽诱导和不定根诱导的影响.结果表明：6-BA是影响‘香宝’愈伤组织诱导的主要因素,各因素影响的主次关系为6-BA>2,4-D>NAA>KT,最适愈伤诱导的培养基为 MS+6-BA 2 mg/L+2,4-D 2 mg/L+NAA 0.3 mg/L,愈伤诱导率为81.28%；6-BA 是影响愈伤增殖的主要因素,各因素影响的主次关系为6-BA>NAA>KT>2,4-D,愈伤组织增殖的最佳配方为 MS+6-BA 1 mg/L +KT 1 mg/L+2,4-D 0.2 mg/L+ NAA 0.05 mg/L,愈伤增值倍数为5.271；GA 是影响不定芽诱导的主要因素,各因素影响的主次关系为 GA>6-BA>NAA,最适不定芽诱导的培养基为 MS+6-BA 1 mg/L+NAA 0.1 mg/L +GA 0.5 mg/L,不定芽诱导率为78.21%；IAA是影响不定根诱导的主要因素,因素影响的主次关系为 IAA>MS>蔗糖>NAA,最适不定根诱导的培养基为1/2 MS+ NAA 0.3 mg/L+蔗糖20 g/L,不定根诱导率为94.4%.
以萱草‘香寶’的花託為外植體,用正交試驗法研究瞭不同激素及其不同濃度對愈傷組織誘導、愈傷組織增殖、不定芽誘導和不定根誘導的影響.結果錶明：6-BA是影響‘香寶’愈傷組織誘導的主要因素,各因素影響的主次關繫為6-BA>2,4-D>NAA>KT,最適愈傷誘導的培養基為 MS+6-BA 2 mg/L+2,4-D 2 mg/L+NAA 0.3 mg/L,愈傷誘導率為81.28%；6-BA 是影響愈傷增殖的主要因素,各因素影響的主次關繫為6-BA>NAA>KT>2,4-D,愈傷組織增殖的最佳配方為 MS+6-BA 1 mg/L +KT 1 mg/L+2,4-D 0.2 mg/L+ NAA 0.05 mg/L,愈傷增值倍數為5.271；GA 是影響不定芽誘導的主要因素,各因素影響的主次關繫為 GA>6-BA>NAA,最適不定芽誘導的培養基為 MS+6-BA 1 mg/L+NAA 0.1 mg/L +GA 0.5 mg/L,不定芽誘導率為78.21%；IAA是影響不定根誘導的主要因素,因素影響的主次關繫為 IAA>MS>蔗糖>NAA,最適不定根誘導的培養基為1/2 MS+ NAA 0.3 mg/L+蔗糖20 g/L,不定根誘導率為94.4%.
이훤초‘향보’적화탁위외식체,용정교시험법연구료불동격소급기불동농도대유상조직유도、유상조직증식、불정아유도화불정근유도적영향.결과표명：6-BA시영향‘향보’유상조직유도적주요인소,각인소영향적주차관계위6-BA>2,4-D>NAA>KT,최괄유상유도적배양기위 MS+6-BA 2 mg/L+2,4-D 2 mg/L+NAA 0.3 mg/L,유상유도솔위81.28%；6-BA 시영향유상증식적주요인소,각인소영향적주차관계위6-BA>NAA>KT>2,4-D,유상조직증식적최가배방위 MS+6-BA 1 mg/L +KT 1 mg/L+2,4-D 0.2 mg/L+ NAA 0.05 mg/L,유상증치배수위5.271；GA 시영향불정아유도적주요인소,각인소영향적주차관계위 GA>6-BA>NAA,최괄불정아유도적배양기위 MS+6-BA 1 mg/L+NAA 0.1 mg/L +GA 0.5 mg/L,불정아유도솔위78.21%；IAA시영향불정근유도적주요인소,인소영향적주차관계위 IAA>MS>자당>NAA,최괄불정근유도적배양기위1/2 MS+ NAA 0.3 mg/L+자당20 g/L,불정근유도솔위94.4%.
Hemeroallis middendorfii Trautv.et Mey ‘Sweet treasure’was used as explants to study the effect of different hormones and their concentrations on the callus induction,proliferation,adventitious bud and roots induction by orthogonal experiment.The results showed that 6-BA was a key factor on the callus induc-tion,the effect sequence of four hormones was 6-BA>2 ,4-D>NAA>KT,the optimum callus induction medium was MS+6-BA 2 mg/L+2,4-D 2 mg/L+ NAA 0.3 mg/L and the callus induction rate was 81.28%.6-BA was also the main factor influencing callus proliferation,the effect sequence of four hormones was 6-BA>NAA>KT>2,4-D,the optimal callus proliferation medium was MS+6-BA 1 mg/L +KT 1 mg/L+2,4-D 0.2 mg/L+ NAA 0.05 mg/L and the callus proliferation rate was 52.71%.GA was a leading factor on the adventitious bud induc-tion,the effect sequence of four hormones was GA>6-BA>NAA,the optimum medium was MS+6-BA 1 mg/L+NAA 0.1 mg/L+GA 0.5 mg/L,and the adventitious bud induction rate was 78.21%;IAA was a major factor on the adventitious roots induction,the effect sequence of four hormones was IAA>MS>sucrose>NAA ,the opti-mum adventitious roots induction medium was 1/2 MS+ NAA 0.3 mg/L+sucrose 20 g/L,and the adventitious roots induction rate was 7 8 .2 1%.
