CAJ | 학술논문

背景：成骨细胞与骨髓干细胞共培养后可以诱导骨髓干细胞向成骨细胞分化，成骨细胞与脂肪干细胞共培养是否也能诱导向成骨细胞分化呢？目的：观察脂肪干细胞与成骨细胞共培养后能否向成骨细胞分化。方法：分离新西兰大白兔脂肪干细胞和成骨细胞，待脂肪干细胞生长至3代，成骨细胞生长至2代时，进行共培养。根据培养时血清浓度不同分为10%胎牛血清共培养组和5%胎牛血清共培养组，共培养14 d。结果与结论：共培养7 d后，2组脂肪干细胞均出现部分变圆。14 d后，脂肪干细胞高度分化与成熟成骨细胞相似，碱性磷酸酶染色阳性、茜素红染色阳性，其Ⅰ型胶原和骨钙素mRNA表达均增高，以10%胎牛血清培养组更为明显。提示脂肪干细胞与成骨细胞经过共培养后可以向成骨细胞分化，高浓度血清培养可以促进诱导作用。
배경：성골세포여골수간세포공배양후가이유도골수간세포향성골세포분화，성골세포여지방간세포공배양시부야능유도향성골세포분화니？목적：관찰지방간세포여성골세포공배양후능부향성골세포분화。방법：분리신서란대백토지방간세포화성골세포，대지방간세포생장지3대，성골세포생장지2대시，진행공배양。근거배양시혈청농도불동분위10%태우혈청공배양조화5%태우혈청공배양조，공배양14 d。결과여결론：공배양7 d후，2조지방간세포균출현부분변원。14 d후，지방간세포고도분화여성숙성골세포상사，감성린산매염색양성、천소홍염색양성，기Ⅰ형효원화골개소mRNA표체균증고，이10%태우혈청배양조경위명현。제시지방간세포여성골세포경과공배양후가이향성골세포분화，고농도혈청배양가이촉진유도작용。
BACKGROUND:After co-culture with osteoblasts, bone marrow stem cells can be induced to differentiate into osteoblasts. Whether adipose-derived stem cells co-cultured with osteoblasts can differentiate into osteoblasts or not? OBJECTIVE:To observe whether adipose-derived stem cells co-cultured with osteoblasts can differentiate into osteoblasts. METHODS:Adipose-derived stem cells and osteoblasts were isolated from New Zealand white rabbits. Then, passage 3 adipose-derived stem cells were co-cultured with passage 2 osteoblasts in 10%or 5%fetal bovine serum for 14 days. RESULTS AND CONCLUSION:After 7 days of co-culture, some adipose-derived stem cells became round in the two groups. After 14 days of co-culture, adipose-derived stem cells highly differentiated and differentiated cells were similar to mature osteoblasts that were positive for alkaline phosphatase staining and alizarin red staining. The mRNA expression of type I col agen and osteocalcin increased in both two group, especial y in the 10%fetal bovine serum group. These findings indicate that adipose-derived stem cells co-cultured with osteoblasts can differentiate into osteoblasts induced by high-concentration serum culture.