CAJ | 학술논문

应用半定量RT-PCR法，研究苯酚对红鳍东方鲀（Takifugu rubripes）幼鱼肝胰脏CuZn-SOD和Mn-SOD基因相对表达量的影响。试验鱼饲养在苯酚浓度为0、0.18、0.32、0.56、1.00和1.35 mg/L的海水中，分别在苯酚暴露前和暴露4、8、12和16 d后取样。结果表明，在苯酚暴露前，鱼体肝胰脏中CuZn-SOD和Mn-SOD基因均有基础表达。在整个暴露时间（16 d）下，CuZn-SOD和Mn-SOD mRNA相对表达量呈现出逐渐上调的趋势，即苯酚诱导了两种基因的表达。在苯酚暴露第4天时，各浓度处理组CuZn-SOD mRNA和Mn-SOD mRNA的相对表达量均达峰值，显著高于暴露前水平（P＜0.05）。
응용반정량RT-PCR법，연구분분대홍기동방돈（Takifugu rubripes）유어간이장CuZn-SOD화Mn-SOD기인상대표체량적영향。시험어사양재분분농도위0、0.18、0.32、0.56、1.00화1.35 mg/L적해수중，분별재분분폭로전화폭로4、8、12화16 d후취양。결과표명，재분분폭로전，어체간이장중CuZn-SOD화Mn-SOD기인균유기출표체。재정개폭로시간（16 d）하，CuZn-SOD화Mn-SOD mRNA상대표체량정현출축점상조적추세，즉분분유도료량충기인적표체。재분분폭로제4천시，각농도처리조CuZn-SOD mRNA화Mn-SOD mRNA적상대표체량균체봉치，현저고우폭로전수평（P＜0.05）。
The semi-quantitative RT-PCR method was applied to test the expression of CuZn-SOD gene and Mn-SOD gene in Takifugu rubripeshepatopancreas after phenol exposure. Fish was cultured in seawater containing0, 0.18, 0.32, 0.56, 1.00 and 1.35 mg/L phenol, respectively, and was sampled before phenol exposure and after 4, 8, 12 and 16 d phenol exposure. The results show that basal expression of CuZn-SOD gene and Mn-SOD gene were detected in fish before phenol exposure. The relative expression levels of CuZn-SOD and Mn-SOD mRNA showed a trend of gradual up-regulation throughout the 16 days phenol exposure andthe phenol induced the expression of CuZn-SOD and Mn-SOD gene. The mRNA expression level in each phenol exposure group peaked at the fourth day and was significantly higher than that of the phenol pre-exposure level(P<0.05).