CAJ | 학술논문

背景：外泌体是间充质干细胞分泌的膜性小囊泡，越来越多的研究表明，间充质干细胞可能通过旁分泌外泌体而发挥对组织损伤的修复作用，目前人脐血间充质干细胞来源的外泌体分离鉴定尚未见报道。目的：分离纯化人脐血间充质干细胞来源的外泌体，并鉴定其生物学特性。方法：收集人脐血间充质干细胞培养上清，采用超滤及梯度离心法分离并纯化外泌体，通过透射电镜观察其形态，流式细胞术检测其表面标志 CD63、CD81、CD90、CD73、CD105、CD29、CD166。结果与结论：人脐血来源的间充质干细胞能够分泌外泌体，电镜下呈椭圆或碟状的囊泡结构，直径40-100 nm。外泌体表达其共性表达标志 CD63、CD81及间充质干细胞表面黏附分子 CD90、CD73、CD105、CD29、CD166。结果表明采用超滤及梯度离心法可以成功从间充质干细胞培养上清中分离纯化外泌体，间充质干细胞分泌的外泌体具有外泌体的胞膜蛋白组分。
배경：외비체시간충질간세포분비적막성소낭포，월래월다적연구표명，간충질간세포가능통과방분비외비체이발휘대조직손상적수복작용，목전인제혈간충질간세포래원적외비체분리감정상미견보도。목적：분리순화인제혈간충질간세포래원적외비체，병감정기생물학특성。방법：수집인제혈간충질간세포배양상청，채용초려급제도리심법분리병순화외비체，통과투사전경관찰기형태，류식세포술검측기표면표지 CD63、CD81、CD90、CD73、CD105、CD29、CD166。결과여결론：인제혈래원적간충질간세포능구분비외비체，전경하정타원혹설상적낭포결구，직경40-100 nm。외비체표체기공성표체표지 CD63、CD81급간충질간세포표면점부분자 CD90、CD73、CD105、CD29、CD166。결과표명채용초려급제도리심법가이성공종간충질간세포배양상청중분리순화외비체，간충질간세포분비적외비체구유외비체적포막단백조분。
BACKGROUND:Exosomes are membrane vesicles secreted by mesenchymal stem cells. Increasing studies have shown that mesenchymal stem cells can secrete exosomes via paracrine function to play a role in tissue injury. However, reports on how to isolate and identify exosomes derived from human umbilical cord blood mesenchymal stem cells are few. OBJECTIVE:To extract, purify and identify exosomes derived from human umbilical cord blood mesenchymal stem cells. METHODS:The cellculture supernatant of human umbilical cord blood mesenchymal stem cells was col ected. Exosome was extracted and purified with ultrafiltration and gradient centrifugation methods. The morphology of exosome was observed by transmission electronic microscope, and the expressions of CD63, CD81, CD90, CD73, CD105, CD29, and CD166 in exosome of mesenchymal stem cells were analyzed by fluorescent activated cellsorting. RESULTS AND CONCLUSION:Mesenchymal stem cells from human umbilical cord blood secreted exosome which exhibited el iptic or saucer-like shape and its diameter ranged from 40 to 100 nm with membrane structure. Exosome could express the common surface adhesion molecules CD63, CD81 and the surface adhesion molecules CD90, CD73, CD105, CD29, CD166 of mesenchymal stem cells. These findings indicate that exosome may be secreted by mesenchymal stem cells of human umbilical cord blood, which contains plasma membrane proteins of umbilical cord blood mesenchymal stem cells.