CAJ | 학술논문

干细胞%骨髓干细胞%多发性骨髓瘤%骨髓间充质干细胞%细胞培养上清液%细胞迁移%直接成管能力%血管生成能力%脐静脉内皮细胞%血管生成因子%活动期%缓解期%国家自然科学基金榦細胞%骨髓榦細胞%多髮性骨髓瘤%骨髓間充質榦細胞%細胞培養上清液%細胞遷移%直接成管能力%血管生成能力%臍靜脈內皮細胞%血管生成因子%活動期%緩解期%國傢自然科學基金
간세포%골수간세포%다발성골수류%골수간충질간세포%세포배양상청액%세포천이%직접성관능력%혈관생성능력%제정맥내피세포%혈관생성인자%활동기%완해기%국가자연과학기금
bone marrow%mesenchymal stem cells%multiple myeloma%cellmovement

背景：活动期和缓解期多发性骨髓瘤患者骨髓间充质干细胞的血管生成能力有无差异目前尚不清楚。目的：观察多发性骨髓瘤活动期及缓解期骨髓间充质干细胞促血管生成能力的差异。方法：抽取13例多发性骨髓瘤患者治疗前和4个周期治疗后的骨髓，分离培养出骨髓间充质干细胞，采用ELISA 方法测定骨髓间充质干细胞培养上清液中血管内皮生长因子、肝细胞生长因子、碱性成纤维细胞生长因子的浓度；MTT检测骨髓间充质干细胞培养上清液对脐静脉内皮细胞增殖能力的影响；通过Transwel 小室观察骨髓间充质干细胞培养上清液对脐静脉内皮细胞运动能力的影响；通过人工基底膜实验观察骨髓间充质干细胞体外促血管生成和成管能力。结果与结论：活动期多发性骨髓瘤患者骨髓间充质干细胞培养上清中血管内皮生长因子、肝细胞生长因子、碱性成纤维细胞生长因子浓度明显高于缓解期(P<0.05)；骨髓瘤患者骨髓间充质干细胞上清对脐静脉内皮细胞增殖作用呈剂量依赖性，活动期明显强于缓解期(P<0.05)；活动期骨髓瘤患者骨髓间充质干细胞上清对脐静脉内皮细胞迁移作用明显高于缓解期(P<0.05)；骨髓瘤患者骨髓间充质干细胞体外具有明显成血管作用，活动期明显强于缓解期(P<0.05)。结果提示多发性骨髓瘤活动期骨髓间充质干细胞促血管生成能力明显大于缓解期。
배경：활동기화완해기다발성골수류환자골수간충질간세포적혈관생성능력유무차이목전상불청초。목적：관찰다발성골수류활동기급완해기골수간충질간세포촉혈관생성능력적차이。방법：추취13례다발성골수류환자치료전화4개주기치료후적골수，분리배양출골수간충질간세포，채용ELISA 방법측정골수간충질간세포배양상청액중혈관내피생장인자、간세포생장인자、감성성섬유세포생장인자적농도；MTT검측골수간충질간세포배양상청액대제정맥내피세포증식능력적영향；통과Transwel 소실관찰골수간충질간세포배양상청액대제정맥내피세포운동능력적영향；통과인공기저막실험관찰골수간충질간세포체외촉혈관생성화성관능력。결과여결론：활동기다발성골수류환자골수간충질간세포배양상청중혈관내피생장인자、간세포생장인자、감성성섬유세포생장인자농도명현고우완해기(P<0.05)；골수류환자골수간충질간세포상청대제정맥내피세포증식작용정제량의뢰성，활동기명현강우완해기(P<0.05)；활동기골수류환자골수간충질간세포상청대제정맥내피세포천이작용명현고우완해기(P<0.05)；골수류환자골수간충질간세포체외구유명현성혈관작용，활동기명현강우완해기(P<0.05)。결과제시다발성골수류활동기골수간충질간세포촉혈관생성능력명현대우완해기。
BACKGROUND:Recent studies have found that bone marrow mesenchymal stem cells play an important role in the development of multiple myeloma, especial y for angiogenesis, but whether the pro-angiogenesis ability of bone marrow mesenchymal stem cells in multiple myeloma in active and remissive state is different is yet unclear. OBJECTIVE:To investigate the difference in the pro-angiogenesis ability of bone marrow mesenchymal stem cells in multiple myeloma in active and remissive state. METHODS:Bone marrow samples were extracted from 13 patients with multiple myeloma before treatment and after four therapeutic cycles to isolate bone marrow mesenchymal stem cells. ELISA assay was used to detect levels of vascular endothelial growth factor, hepatocyte growth factor and basic fibroblast growth factor in the supernatant of bone marrow mesenchymal stem cells. The proliferative ability and movement of human umbilical vein endothelial cells cultured in the supernatant of bone marrow mesenchymal stem cells were detected by MTT assay and Transwel assay, respectively. The pro-angiogenesis ability of bone marrow mesenchymal stem cells was measured by Matrigel in vitro. RESULTS AND CONCLUSION:The levels of vascular endothelial growth factor, hepatocyte growth factor and basic fibroblast growth factor in the cellsupernatant were significantly higher in active myeloma than those in remissive myeloma (P<0.05). The supernatant of bone marrow mesenchymal stem cells of active myeloma more significantly promoted the proliferation, migration and blood vessel formation of human umbilical vein endothelial cells than that of remissive myeloma in a dose-dependent manner (P<0.05). These findings indicate that bone marrow mesenchymal stem cells from active myeloma have stronger pro-angiogenesis ability than those from remissive myeloma.