人参研究
人參研究
인삼연구
RENSHEN YANJIU
2014年
3期
39-42
,共4页
苦参%发根农杆菌%诱导率%毛状根%氧化苦参碱
苦參%髮根農桿菌%誘導率%毛狀根%氧化苦參堿
고삼%발근농간균%유도솔%모상근%양화고삼감
Sophora flavescens Ait.%Agrobacterium rhizogenes%Hairy roots%Induction rate%Oxymatrine
本文主要利用三种发根农杆菌 A4、15834、R1601诱导苦参产生毛状根，初步为建立苦参的毛状根培养体系提供参考。本实验采用共培养法，考察了不同外植体、菌种、培养时间对苦参毛状根诱导率的影响，并对苦参毛状根中氧化苦参碱进行初步的含量测定。研究结果表明，苦参毛状根的最佳诱导条件是以生长1周的苦参幼芽为外植体，R1601为菌株，添加100μmol/l 的乙酰丁香酮，侵染20 min，(25±1)℃暗处诱导，最终测定诱导率最高为27.6%，存活率为45.8%，而苦参毛状根中氧化苦参碱的富集量为1.68mg/g（苦参毛状根干重），未检测到苦参碱。
本文主要利用三種髮根農桿菌 A4、15834、R1601誘導苦參產生毛狀根，初步為建立苦參的毛狀根培養體繫提供參攷。本實驗採用共培養法，攷察瞭不同外植體、菌種、培養時間對苦參毛狀根誘導率的影響，併對苦參毛狀根中氧化苦參堿進行初步的含量測定。研究結果錶明，苦參毛狀根的最佳誘導條件是以生長1週的苦參幼芽為外植體，R1601為菌株，添加100μmol/l 的乙酰丁香酮，侵染20 min，(25±1)℃暗處誘導，最終測定誘導率最高為27.6%，存活率為45.8%，而苦參毛狀根中氧化苦參堿的富集量為1.68mg/g（苦參毛狀根榦重），未檢測到苦參堿。
본문주요이용삼충발근농간균 A4、15834、R1601유도고삼산생모상근，초보위건립고삼적모상근배양체계제공삼고。본실험채용공배양법，고찰료불동외식체、균충、배양시간대고삼모상근유도솔적영향，병대고삼모상근중양화고삼감진행초보적함량측정。연구결과표명，고삼모상근적최가유도조건시이생장1주적고삼유아위외식체，R1601위균주，첨가100μmol/l 적을선정향동，침염20 min，(25±1)℃암처유도，최종측정유도솔최고위27.6%，존활솔위45.8%，이고삼모상근중양화고삼감적부집량위1.68mg/g（고삼모상근간중），미검측도고삼감。
n this paper,hairy root of Sophora flavescens Ait.were induced by the infection of third Agrobacterium rhizogenes strains 15834,R1601,A4 and an in vitro culture system of the hairy roots was newly established.Hairy roots were induced by co -culture.Effects of explants, Agrobacterium rhizogenes,culture time on growth of hairy root were studied and Determination of Oxymatrine in Sophora flavescens Ait.The research shows that the highest induction frequency was obtained from young shoot with one week which were induced by R1601 for 10mins ,and increase of 100 μmol /L acetosyringone. The transformation frequency was 27.6%,annual survival rate was 45.8%. Hairy roots of Oxymatrine enrichment amount 1.68mg/g ,Total matrine content was not determined.
