CAJ | 학술논문

目的：研究四种生物膜的表面形态及生物相容性，比较它们对 MC3 T3-E1细胞粘附增殖能力的影响，及成膜材料与制备方法之间的交互作用。方法：以聚己内酯[poly（ε-caprolactone），PCL]与聚乳酸-羟基乙酸[poly（lactic-co-glycolic acid）， PLGA]作为膜材料，浇铸及电纺两种方法分别制备生物膜，使用场发射扫描电子显微镜观察四种膜表面结构，CCK-8法检测膜上 MC3T3-E1细胞1、3、7 d增殖量，激光共聚焦显微镜评价细胞在材料表面的粘附情况，实时荧光定量 PCR反应检测粘附相关基因。结果：扫描电镜观察到浇铸膜表面具有孔隙结构，电纺膜表面电纺丝交织成网；CCK-8检测中3 d、7 d 时 PLGA 膜上细胞增殖量大于 PCL膜（P＜0．05），与制备方法无关（P＞0．05）；激光共聚焦显微镜观察到除 PCL 电纺膜表面细胞铺展不佳外，其余膜上细胞生长良好；实时荧光定量 PCR检测发现 PCL浇铸膜上细胞整合素基因表达量高于其他组（P＜0．05），且不同材料与制备方法间存在交互作用。结论：PLGA膜有利于 MC3T3-E1细胞增殖，而 PCL 浇铸膜适合细胞粘附。因此，相对单一材料或成膜方式，PLGA与 PCL及浇铸、电纺法的混合膜可成为以后发展的方向。
목적：연구사충생물막적표면형태급생물상용성，비교타문대 MC3 T3-E1세포점부증식능력적영향，급성막재료여제비방법지간적교호작용。방법：이취기내지[poly（ε-caprolactone），PCL]여취유산-간기을산[poly（lactic-co-glycolic acid）， PLGA]작위막재료，요주급전방량충방법분별제비생물막，사용장발사소묘전자현미경관찰사충막표면결구，CCK-8법검측막상 MC3T3-E1세포1、3、7 d증식량，격광공취초현미경평개세포재재료표면적점부정황，실시형광정량 PCR반응검측점부상관기인。결과：소묘전경관찰도요주막표면구유공극결구，전방막표면전방사교직성망；CCK-8검측중3 d、7 d 시 PLGA 막상세포증식량대우 PCL막（P＜0．05），여제비방법무관（P＞0．05）；격광공취초현미경관찰도제 PCL 전방막표면세포포전불가외，기여막상세포생장량호；실시형광정량 PCR검측발현 PCL요주막상세포정합소기인표체량고우기타조（P＜0．05），차불동재료여제비방법간존재교호작용。결론：PLGA막유리우 MC3T3-E1세포증식，이 PCL 요주막괄합세포점부。인차，상대단일재료혹성막방식，PLGA여 PCL급요주、전방법적혼합막가성위이후발전적방향。
Objective:To study the surface characteristics and biocompatibility of four kinds of biological membranes in order to compare their applications of cell adhesion and proliferation,as well as interaction between materials and membrane types.Methods:By casting or electrospinning,we fabricated poly(ε-caprolactone)(PCL)membranes and poly(lactic-co-glycolic acid)(PLGA)mem-branes.Field emission scanning electron microscope (FESEM)was used to detect the surface microstructure of the membranes.After Cell line MC3T3-E1 was seeding to all membranes,we tested cell proliferation of 1 ,3,7days by Cell counting Kit-8 (CCK-8)assay, while estimating cell adhesion by confocal laser scanning microscope (CLSM)and real-time quantitative polymerase chain reaction (RT-qPCR).Results:FESEM pictures showed pores on cast membranes,while interwoven fibers were observed on electrospun mem-brane surfaces.CCK-8 tests showed MC3T3-E1 cells prefer two PLGA groups than PCL groups(P<0.05),no matter how the mem-branes were fabricated(P>0.05 ).Despite PCL electrospun membranes,cells were observed spreading well on other membranes by CLSM.RT-qPCR tests showed there are interactions between materials and membrane types,and PCL cast membrane group showed highest integrin gene expression(P<0.05).Conclusions:PLGA membranes contributed to MC3T3-E1 cell proliferation,while PCL cast membranes were suitable for cellular adhesion.Other than singly using one kind of material or fabrication method,combination use of PLGA,PCL by casting or electrospinning will become the trend to fabricate biological membranes.