CAJ | 학술논문

背景：移植的肌腱必须具有良好的生物力学性能，才能有效避免缝合时肌腱吻合端的撕裂，减轻肌腱愈合过程中粘连程度。目的：探讨移植修复材料不同制备方法对异体肌腱生物学性能的影响。方法：将48只健康雄性来亨鸡依据随机数字表法分为均分为3组，切取3组鸡一侧第三趾屈趾浅深肌腱，玻璃化组、化学萃取组分别采用玻璃化法与化学萃取法处理肌腱，空白对照组肌腱不做任何处理。取3组一部分肌腱进行生物力学检测；另一部分进行异体移植，移植1，2，3，6周检测外周血CD4+、CD8+T淋巴细胞数。结果与结论：玻璃化法可保留部分原有肌腱细胞，化学萃取法不能。3组肌腱拉伸断裂强度、拉伸断裂功耗及拉伸断裂延伸率比较差异无显著性意义(P >0.05)。移植后1，2周末，3组间CD4+、CD8+、CD4+/CD8+比较差异有显著性意义(P <0.05)；移植后3，6周末，玻璃化组、化学萃取组CD4+、CD8+、CD4+/CD8+均明显少于空白对照组(P<0.05)，玻璃化组和化学萃取组间CD4+、CD8+、CD4+/CD8+比较差异无显著性意义(P>0.05)。表明采用玻璃化法和化学萃取法在有效保留肌腱生物力学性能的基础上，可显著降低肌腱的免疫原性。
배경：이식적기건필수구유량호적생물역학성능，재능유효피면봉합시기건문합단적시렬，감경기건유합과정중점련정도。목적：탐토이식수복재료불동제비방법대이체기건생물학성능적영향。방법：장48지건강웅성래형계의거수궤수자표법분위균분위3조，절취3조계일측제삼지굴지천심기건，파리화조、화학췌취조분별채용파리화법여화학췌취법처리기건，공백대조조기건불주임하처리。취3조일부분기건진행생물역학검측；령일부분진행이체이식，이식1，2，3，6주검측외주혈CD4+、CD8+T림파세포수。결과여결론：파리화법가보류부분원유기건세포，화학췌취법불능。3조기건랍신단렬강도、랍신단렬공모급랍신단렬연신솔비교차이무현저성의의(P >0.05)。이식후1，2주말，3조간CD4+、CD8+、CD4+/CD8+비교차이유현저성의의(P <0.05)；이식후3，6주말，파리화조、화학췌취조CD4+、CD8+、CD4+/CD8+균명현소우공백대조조(P<0.05)，파리화조화화학췌취조간CD4+、CD8+、CD4+/CD8+비교차이무현저성의의(P>0.05)。표명채용파리화법화화학췌취법재유효보류기건생물역학성능적기출상，가현저강저기건적면역원성。
BACKGROUND:The transplanted tendon must have good biomechanical properties, in order to effectively avoid tendon tear at the anastomosis end during suturing and reduce adhesion of tendon during healing process. OBJECTIVE:To investigate the effects of different methods for preparation of graft materials on the biological properties of tendon al ograft. METHODS:Forty-eight healthy male Leghorns were randomly divided into three groups:vitrification group, chemical extraction group, and control group. Unilateral superficial and deep flexor tendon of the third toe was subjected to vitrification, chemical extraction and no treatment in the three groups, respectively. A part of tendon was taken for biomechanical testing, and the other part was for al ogeneic transplantation. After 1, 2, 3, 6 weeks, peripheral blood CD4+, CD8+T lymphocytes were counted. RESULTS AND CONCLUSION:Vitrification could partial y retain the original tendon cells, but the chemical extraction method could not. Tensile strength for tendon rupture, tensile fracture power and tensile elongation at break were not statistical y significant among three groups (P>0.05). At the end of 1 and 2 weeks after transplantation, CD4+, CD8+, CD4+/CD8+difference was significant among the three groups (P<0.05);at the end of 3 and 6 weeks after transplantation, CD4+, CD8+, CD4+/CD8+were significantly less in the vitrification and chemical extraction groups than the control group (P<0.05), but no difference was found between the vitrification group and chemical extraction group (P>0.05). These findings indicate that the vitrification and chemical extraction methods can significantly reduce immunogenicity of the tendon based on effective retention of biomechanical properties of the tendon.