CAJ | 학술논문

背景：有研究显示纤维蛋白胶可促进成肌细胞移植物的保持和生存，减少梗死范围并在梗死区诱导新生血管化。目的：了解内皮祖细胞经可降解材料纤维蛋白胶移植到大鼠梗死心肌后的血管再生情况。方法：将27只SD大鼠随机均分为3组，非心肌梗死组9只、心肌梗死即刻移植组9只与心肌梗死1周移植组9只。每个大组又再分为两个亚组，即移植人脐带源内皮祖细胞-纤维蛋白胶复合物的实验组与移植纤维蛋白胶的对照组。移植后3，8周处死，通过显微镜、免疫组织化学和心脏超声观察其在梗死心肌的血管再生和心功能改善情况。结果与结论：显微镜观察到，实验组大鼠心脏和胸部之间有一些疏松的结缔组织，而其与对照组之间无明显差异。组织和免疫学观察发现，各实验组和对照组的心脏结构不易区分且相对正常，未发现血管瘤、血管畸形和肿瘤等。血管测量结果显示实验组和对照组之间，以及各实验组之间均无差异，并且实验组和对照组之间心功能检查也没有统计学意义。此次研究内皮祖细胞结果没有阳性表现，将修改并提高细胞通过纤维蛋白基质传递的方法策略，确信细胞传递系统提供的有益性和有效性将会进一步得到证实。
배경：유연구현시섬유단백효가촉진성기세포이식물적보지화생존，감소경사범위병재경사구유도신생혈관화。목적：료해내피조세포경가강해재료섬유단백효이식도대서경사심기후적혈관재생정황。방법：장27지SD대서수궤균분위3조，비심기경사조9지、심기경사즉각이식조9지여심기경사1주이식조9지。매개대조우재분위량개아조，즉이식인제대원내피조세포-섬유단백효복합물적실험조여이식섬유단백효적대조조。이식후3，8주처사，통과현미경、면역조직화학화심장초성관찰기재경사심기적혈관재생화심공능개선정황。결과여결론：현미경관찰도，실험조대서심장화흉부지간유일사소송적결체조직，이기여대조조지간무명현차이。조직화면역학관찰발현，각실험조화대조조적심장결구불역구분차상대정상，미발현혈관류、혈관기형화종류등。혈관측량결과현시실험조화대조조지간，이급각실험조지간균무차이，병차실험조화대조조지간심공능검사야몰유통계학의의。차차연구내피조세포결과몰유양성표현，장수개병제고세포통과섬유단백기질전체적방법책략，학신세포전체계통제공적유익성화유효성장회진일보득도증실。
BACKGROUND:Studies have shown that fibrin glue can promote the survival of myoblast grafts, reduce infarct size and induce neovascularization of infarct zone. OBJECTIVE:To understand the condition of revascularization of infarcted heart muscle using endothelial progenitor cells combined with degradable fibrin glue materials. METHODS:A total of 27 Sprague-Dawley rats were randomized into three groups, 9 rats in each group:non-myocardial infarction group, immediate transplantation group and 1-week post-infarction transplantation group. Then, these three groups were sub-grouped into two groups, respectively:endothelial progenitor cells+fibrin glue group (experimental group) and fibrin glue group (control group). At 3 and 8 weeks after transplantation, the rats were sacrificed in each group. The revascularization and function of infracted heart muscle were observed by microscope, immunohistochemistry and echocardiography. RESULTS AND CONCLUSION:Under the microscope, there were some lax connective tissues between the heart and chest in the experimental groups, but no difference existed between the experimental and control groups. The heart structure was normal relatively and difficult to be distinguished between the experimental and control groups histological y and immunological y, and there was no angeioma, vascular malformation and tumor. The number of revascularization of heart muscle showed no difference between experimental and control groups as wel as between different experimental groups. Additional y, there was no significant difference in cardiac function between experimental and control groups. Although there are no positive results of endothelial progenitor cells, we wil modify and improve the strategy and believe that the celldelivery system is of benefit and efficacy.