CAJ | 학술논문

背景：拔牙后会发生牙槽嵴的萎缩和吸收，对于采用何种方法或者何种材料能更有效预防拔牙后牙槽嵴的萎缩，目前还是难以彻底解决的技术问题。目的：观察纳米壳聚糖骨形态发生蛋白水凝胶预防牙槽嵴萎缩的效果。方法：取54只SD大鼠，拔除左侧中切牙制作剩余牙槽嵴吸收模型，随机分均分为3组，实验组在拔牙窝内注入纳米壳聚糖骨形态发生蛋白水凝胶，对照组在拔牙窝内注入纳米壳聚糖水凝胶，空白组不注入任何材料。术后3，6，9周完整分离下颌骨，高频钼靶X射线测量剩余牙槽嵴高度，并进行组织学观察。结果与结论：术后3，6，9周各组牙槽嵴顶均有不同程度吸收，实验组吸收程度较对照组、空白组轻(P<0.05)。术后3周时，空白组可见出血及大量炎性细胞，成骨较少；对照组牙槽骨可见少量骨样组织及淋巴细胞，实验组可见新生骨组织及少量炎性细胞。术后6周时，实验组有较明显新骨生成，对照组有少量成骨，空白组成骨欠活跃。术后9周时，差异性更加明显，实验组有大量新骨生成，成骨活跃；对照组及空白组有少量新骨生成。结果表明纳米壳聚糖骨形态发生蛋白水凝胶能有效预防拔牙后剩余牙槽嵴萎缩。
배경：발아후회발생아조척적위축화흡수，대우채용하충방법혹자하충재료능경유효예방발아후아조척적위축，목전환시난이철저해결적기술문제。목적：관찰납미각취당골형태발생단백수응효예방아조척위축적효과。방법：취54지SD대서，발제좌측중절아제작잉여아조척흡수모형，수궤분균분위3조，실험조재발아와내주입납미각취당골형태발생단백수응효，대조조재발아와내주입납미각취당수응효，공백조불주입임하재료。술후3，6，9주완정분리하합골，고빈목파X사선측량잉여아조척고도，병진행조직학관찰。결과여결론：술후3，6，9주각조아조척정균유불동정도흡수，실험조흡수정도교대조조、공백조경(P<0.05)。술후3주시，공백조가견출혈급대량염성세포，성골교소；대조조아조골가견소량골양조직급림파세포，실험조가견신생골조직급소량염성세포。술후6주시，실험조유교명현신골생성，대조조유소량성골，공백조성골흠활약。술후9주시，차이성경가명현，실험조유대량신골생성，성골활약；대조조급공백조유소량신골생성。결과표명납미각취당골형태발생단백수응효능유효예방발아후잉여아조척위축。
BACKGROUND:Absorption and atrophy of the alveolar ridge always happen after tooth extraction. There are stil a lot of difficulties to be worked out to prevent the atrophy of the alveolar ridge. OBJECTIVE:To investigate the effect of the nano-chitosan/bone morphogenetic protein hydrogel on preventing absorption of the residual alveolar ridge of the rats. METHODS:Total y 54 Sprague-Dawley rats were divided randomly and equal y into three groups. A model of residual alveolar ridge was made by extraction of the left central incisor. Nano-chitosan bone morphogenetic protein hydrogel was implanted into the rat’s alveolar fossa of the experimental group;the alveolar fossa of the control group was implanted with nano-chitosan hydrogel;the alveolar fossa of the blank group was implanted with nothing. Rat’s mandible was ful y dissected after 3, 6, 9 weeks, respectively. Molybdenum target X-ray examination and pathological observation were evaluated. RESULTS AND CONCLUSION:At weeks 3, 6, 9 postoperation, the residual alveolar ridge was absorbed to different degrees, which was milder in the experimental group than the cotrol group and blank group (P<0.05). After 3 weeks, in the blank group, less new bone formed, but bleeding and a large amount of inflammatory cells were visible; in the control group, a few of osteoid tissues and lymphocytes were found; in the experimental group,new bone formed and a small amount of inflammatory cells were observed. After 6 weeks, new bone formed obviously in the experimental group, a little new bone formed in the control group, but less new bone formed in the blank group. After 9 weeks, a large amount of new bone formed in the experimental group with good osteogenic ability; only a few of new bone formed in the control and blank groups. Nano-chitosan/bone morphogenetic protein hydrogel can be used to prevent the absorption of the residual alveolar ridge after tooth extraction.