CAJ | 학술논문

背景：纯钛阳极氧化改性后形成的纳米结构与骨组织具有良好的生物相容性。目的：观察纯钛表面纳米孔结构的形貌和物相构成，以及其对MC3T3-E1小鼠前成骨细胞增殖、黏附等生物学行为和促成骨基因护骨素表达的影响。方法：取纯钛片24份，其中12份仅进行机械抛光，作为对照组；另外12份进行机械抛光后，应用阳极氧化技术在纯钛表面制备纳米孔结构，作为实验组。将小鼠前成骨细胞MC3T3-E1分别接种于两组试件表面，接种7 d后扫描电镜下观察细胞形态，采用MTT法检测细胞增殖情况，绘制生长曲线；同时检测细胞促成骨基因护骨素的表达。结果与结论：阳极氧化后钛片表面形成规格统一的纳米孔结构，但是物相构成并未发生变化。与接种于对照组试件上的成骨细胞相比，实验组试件表面的细胞密度变大，覆盖金属的面积更多，呈现多边形结构，突触向周围移行，可见板状伪足向周围材料伸出；接种第7天时，实验组细胞数目约为对照组的1.4倍，同时纳米孔表面成骨细胞护骨素基因的表达高于对照组(P<0.01)。结果表明阳极氧化后形成纳米孔结构的钛片更有利于成骨细胞的黏附、增殖和护骨素基因的表达，进而促进成骨细胞生长，具有良好的生物相容性。
배경：순태양겁양화개성후형성적납미결구여골조직구유량호적생물상용성。목적：관찰순태표면납미공결구적형모화물상구성，이급기대MC3T3-E1소서전성골세포증식、점부등생물학행위화촉성골기인호골소표체적영향。방법：취순태편24빈，기중12빈부진행궤계포광，작위대조조；령외12빈진행궤계포광후，응용양겁양화기술재순태표면제비납미공결구，작위실험조。장소서전성골세포MC3T3-E1분별접충우량조시건표면，접충7 d후소묘전경하관찰세포형태，채용MTT법검측세포증식정황，회제생장곡선；동시검측세포촉성골기인호골소적표체。결과여결론：양겁양화후태편표면형성규격통일적납미공결구，단시물상구성병미발생변화。여접충우대조조시건상적성골세포상비，실험조시건표면적세포밀도변대，복개금속적면적경다，정현다변형결구，돌촉향주위이행，가견판상위족향주위재료신출；접충제7천시，실험조세포수목약위대조조적1.4배，동시납미공표면성골세포호골소기인적표체고우대조조(P<0.01)。결과표명양겁양화후형성납미공결구적태편경유리우성골세포적점부、증식화호골소기인적표체，진이촉진성골세포생장，구유량호적생물상용성。
BACKGROUND:Nanostructure formation on titanium surface by anodic oxidation has good biocompatibility with bone tissue. OBJECTIVE:To observe the surface morphology and crystal ine constitution of nanopores microstructure on titanium surface formed by anodic oxidation and to further observe its influence on the MC3T3-E1 osteoblast cells’ biological behavior and the gene expression of osteoprotegerin. METHODS:Nanopores forming on titanium surface by anodic oxidation was prepared as experimental group and polished titanium as control group (12 samples for each group). Mouse MC3T3-E1 osteoblasts were co-cultured with polished pure titanium plate group and anodic oxidation nanopores group. After 7 days of inoculation, cellmorphology was observed using scanning electron microscopy, MTT method was used for the cellproliferation test and the growth curve was made. Gene expression of osteoprotegerin was also detected. RESULTS AND CONCLUSION:After anodic oxidation, a homogeneous and uniform array of nanopores formed;however it had no significant influence on the crystal ine phase of the titanium sample surfaces. Titanium surface with nanopore structure was more favorable than polished titanium surface for cellattachment and spreading. cells on the anodized surface with nanopores had higher celldensity and bigger metal coverage area. cells on the nanopores surface also exhibited a polygonal shape with many filopodia extending in al directions. MTT method showed that the anodized nanopore surface had higher cellamount than the as-polished titanium, and the former was about 1.4 times of the latter group after 7 days of culture. The gene expression level of osteoprotegerin in the MC3T3-E1 cells cultured on anodized titanium surface with nanopores was significantly higher than that on the as-polished titanium (P<0.01). The anodic oxidation treatment is more advantageous for the osteoblasts adhesion and gene expression of osteoprotegerin, thereby promoting the growth of osteoblasts.