CAJ | 학술논문

利用常规石蜡切片、HE染色进行显微结构观察并结合形态剂量学分析，研究不同年龄(3月、1岁、3岁、9岁)牦牛睾丸的显微结构，探讨不同发育阶段牦牛睾丸生精小管和间质细胞的发育及其变化规律.结果表明：3月龄牦牛睾丸生精小管细而稀疏，偶见完整的内腔，小管中仅仅是精原细胞和支持细胞，可以看见单个或者成群分布的间质细胞；1岁牦牛生精小管排列略紧密，上皮中有不同发育阶段的生精细胞，间质可见3~4群的间质细胞；3岁牦牛生精小管密集、生精小管外直径增加明显(P<0.05)，上皮细胞层数较其它年龄达到最大值，大量间质细胞出现；9岁牦牛生精小管稀疏，上皮生精细胞和间质细胞均明显减少(P<0.05).3月牦牛龄生精小管内无精子生成；1岁牦牛时有精子的出现，间质细胞数量逐渐增加；3岁牦牛生精小管及间质细胞发育充分，处于生殖旺盛的性成熟阶段；9岁牦牛睾丸生精小管内生精能力明显降低.
이용상규석사절편、HE염색진행현미결구관찰병결합형태제량학분석，연구불동년령(3월、1세、3세、9세)모우고환적현미결구，탐토불동발육계단모우고환생정소관화간질세포적발육급기변화규률.결과표명：3월령모우고환생정소관세이희소，우견완정적내강，소관중부부시정원세포화지지세포，가이간견단개혹자성군분포적간질세포；1세모우생정소관배렬략긴밀，상피중유불동발육계단적생정세포，간질가견3~4군적간질세포；3세모우생정소관밀집、생정소관외직경증가명현(P<0.05)，상피세포층수교기타년령체도최대치，대량간질세포출현；9세모우생정소관희소，상피생정세포화간질세포균명현감소(P<0.05).3월모우령생정소관내무정자생성；1세모우시유정자적출현，간질세포수량축점증가；3세모우생정소관급간질세포발육충분，처우생식왕성적성성숙계단；9세모우고환생정소관내생정능력명현강저.
The development and change characteristics of yak spermatogenic cells and leydig cells of yak in different ages were studied by morphological observation and morphometric analysis.The results showed as follows:The seminiferous tubules were slender in 3-month-old yak,there were spermatogonia and sup-porting cells only and no obvious sparse in lumen and the leydig cells were present single or aggregates. The seminiferous tubules became dense and leydig cells were present 3-4 group in 1-year-old yak.The semi-niferous tubules became dense with their outer diameter and the number of their cell layers increasing,also there was numbers of active spermatogenesis and leydig cells in 3-year-old yak.The seminiferous tubules became sparse and the epithelial layer decreased considerably in 9-year-old yak.In 3-month-old yak,there was no sperm within the seminiferous tubules and spermatogenic abilities until 1-year-old,although there were large amounts of leydig cells,the peaks of spermatogenic abilities and the best reproductive capabili-ties appeared in puberal yak(3-year-old)while spermatogenic abilities gradually decreased in gerontic yak (9-year-old).