CAJ | 학술논문

为了筛选芽胞杆菌发酵菌液Lx-11长期保存的防腐剂，本文探讨了5种化学防腐剂对解淀粉芽胞杆菌Lx-11的抑制作用。通过比较添加不同浓度防腐剂对发酵液菌含量的影响，试验结果表明，5种防腐剂对发酵液菌含量的抑制作用大小的顺序为尼泊金甲酯＝双乙酸钠＞脱氢乙酸＞苯甲酸钠＞山梨酸钾；进一步对山梨酸钾和苯甲酸钠在最适pH范围内用量比较研究，表明山梨酸钾添加量在1‰时，pH值在5.5～6.0时，对解淀粉芽胞杆菌Lx-11菌含量影响最小。通过对15吨罐体中发酵过程Lx-11菌液菌体性状和数量动态的观察，玻片检测表明，解淀粉芽胞杆菌Lx-11在15吨罐体中发酵4 h后进入对数生长阶段，16 h前后菌体开始形成芽孢，发酵18 h后90%以上为芽孢。对发酵生产Lx-11菌液添加1‰含量山梨酸钾，调节pH至6.0左右，放置180 d后，添加山梨酸钾的发酵液含菌量显著高于未加入山梨酸钾的处理，两者含菌量分别为6.3×109和3.5×108 cfu/mL。
위료사선아포간균발효균액Lx-11장기보존적방부제，본문탐토료5충화학방부제대해정분아포간균Lx-11적억제작용。통과비교첨가불동농도방부제대발효액균함량적영향，시험결과표명，5충방부제대발효액균함량적억제작용대소적순서위니박금갑지＝쌍을산납＞탈경을산＞분갑산납＞산리산갑；진일보대산리산갑화분갑산납재최괄pH범위내용량비교연구，표명산리산갑첨가량재1‰시，pH치재5.5～6.0시，대해정분아포간균Lx-11균함량영향최소。통과대15둔관체중발효과정Lx-11균액균체성상화수량동태적관찰，파편검측표명，해정분아포간균Lx-11재15둔관체중발효4 h후진입대수생장계단，16 h전후균체개시형성아포，발효18 h후90%이상위아포。대발효생산Lx-11균액첨가1‰함량산리산갑，조절pH지6.0좌우，방치180 d후，첨가산리산갑적발효액함균량현저고우미가입산리산갑적처리，량자함균량분별위6.3×109화3.5×108 cfu/mL。
To screen preservatives for long-term preservation of Bacillus amyloliquefaciens Lx-11 fermentation broth, this paper discussed five different preservatives inhibition of B. amyloliquefaciens Lx-11. Adding different concentrations of preservatives and assaying bacteria concentration by dilution plate method, our data showed that five different preservatives had inhibitory effect on fermentation broth at different level. They could be ranked in decreasing order of antibacterial activity as follows: methyl paraben=sodium diacetate>dehydrogenation acid>sodium benzoate>potassium sorbate. Compared with sodium benzoate in optimum pH range, when adding 1‰concentration of potassium sorbate and the pH values at 5.5-6.0, potassium sorbate had least effect on B. amyloliquefaciens Lx-11. In dynamic observation of 15 t tank bacteria fermentation process, we observed that the strain after fermentation 4 h reached the logarithmic growth phase, beginning to form spores around 16 h, and more than 90%formed spores after 18 h. Added 1‰potassium sorbate in fermentation broth, and adjusted pH value to around 6.0, after placing 180 d, the amount of bacteria was significantly higher than that without adding potassium sorbate treatment, which were 6.3×109 and 3.5×108 cfu/mL, respectively.