CAJ | 학술논문

莱氏野村菌微菌核已成功实现了液体诱导培养和对其分子发育机理的研究。为实现微菌核的规模化生产，我们通过对不同类型的接种体、不同培养时间的接种体和不同接种量对莱氏野村菌微菌核产量和生物量进行了研究。结果显示，培养24～48 h的菌体能够作为液体一步发酵的有效接种体，微菌核产量均达到1.0×104微菌核/mL。并优化莱氏野村菌微菌核液体30 L发酵工艺，产率达到20%～30%。研究结果为莱氏野村菌微菌核的规模化生产提供了科学数据参考。
래씨야촌균미균핵이성공실현료액체유도배양화대기분자발육궤리적연구。위실현미균핵적규모화생산，아문통과대불동류형적접충체、불동배양시간적접충체화불동접충량대래씨야촌균미균핵산량화생물량진행료연구。결과현시，배양24～48 h적균체능구작위액체일보발효적유효접충체，미균핵산량균체도1.0×104미균핵/mL。병우화래씨야촌균미균핵액체30 L발효공예，산솔체도20%～30%。연구결과위래씨야촌균미균핵적규모화생산제공료과학수거삼고。
Microsclerotia (MS) formation was successfully induced in Nomuraea rileyi (Ascomycetes:Hypocreales) in liquid culture and the molecular mechanism of MS formation had investigated through comparative transcriptome analysis. To determinate the large scale fermentation, the various inoculums, the different culture times of mycelium and conidium inoculum, and the various inoculum size, affecting N. rileyi MS yields and biomass, were investigated. The results showed that N. rileyi MS yields were 1.0×104 MS/mL with inoculums, cultured in 24-48 hours at 25℃and the mycelia could be used as the effective inoculums for one step fermentation. Furthermore, N. rileyi MS yields of fermentation in 30 liters bioreactor were optimized and up to 20%-30%. The results will provide scientific data for N. rileyi MS fermentation in large scale.