CAJ | 학술논문

为分析七星瓢虫滞育相关蛋白质的类别和功能，在滞育调控及滞育后生物学研究的基础上，应用双向电泳、质谱分析（ESI-QUAD-TOF）、生物信息学等蛋白质组学方法，对表达量存在2倍及以上差异且差异显著的蛋白点进行鉴定。应用 Mascot 软件进行数据库检索，根据数据的匹配程度鉴定蛋白质。所得蛋白质中，参与三羧酸循环的两种关键酶呈现差异性表达，其中苹果酸脱氢酶（gi|212508346）呈上调表达，异柠檬酸脱氢酶（gi|21392222）则呈下调表达。苹果酸脱氢酶的增加，推测与滞育状态下的生理需求相关：一方面与昆虫对 NAD 的合成和利用有关，另一方面或是七星瓢虫应对滞育环境条件的一种应激方式，与正常个体的代谢通路相比，滞育个体开启了另外的代谢通路以适应环境条件的改变。异柠檬酸脱氢酶作为三羧酸循环中起关键调节作用的限速酶，在滞育个体中下调表达，或反映了三羧酸循环整体速率的降低，表现在滞育七星瓢虫体内维持了低水平的能量代谢。本文的研究结果，为从蛋白质水平深入揭示七星瓢虫滞育调控及其机理提供一定的参考。
위분석칠성표충체육상관단백질적유별화공능，재체육조공급체육후생물학연구적기출상，응용쌍향전영、질보분석（ESI-QUAD-TOF）、생물신식학등단백질조학방법，대표체량존재2배급이상차이차차이현저적단백점진행감정。응용 Mascot 연건진행수거고검색，근거수거적필배정도감정단백질。소득단백질중，삼여삼최산순배적량충관건매정현차이성표체，기중평과산탈경매（gi|212508346）정상조표체，이저몽산탈경매（gi|21392222）칙정하조표체。평과산탈경매적증가，추측여체육상태하적생리수구상관：일방면여곤충대 NAD 적합성화이용유관，령일방면혹시칠성표충응대체육배경조건적일충응격방식，여정상개체적대사통로상비，체육개체개계료령외적대사통로이괄응배경조건적개변。이저몽산탈경매작위삼최산순배중기관건조절작용적한속매，재체육개체중하조표체，혹반영료삼최산순배정체속솔적강저，표현재체육칠성표충체내유지료저수평적능량대사。본문적연구결과，위종단백질수평심입게시칠성표충체육조공급기궤리제공일정적삼고。
To analyze the categories and functions of the diapause related proteins in ladybird, Coccinella septempunctata, proteomics methods, such as two-dimensional electrophoresis, ESI-QUAD-TOF and bioinformatics, were used to identify the proteins with more than 2 folds of expression quantity. Mascot database search determined two matching proteins which showed different expression and are associated with the Krebs cycle, malate dehydrogenase (gi|212508346) and isocitrate dehydrogenase (gi|21392222), which were up-regulated and down-regulated, respectively. We analyzed the possible reasons and provided a theoretical basis for the diapauses environmental regulation at the protein level.