CAJ | 학술논문

目的：采用RT-PCR检测方法观察皮肤原位再生复原技术（ Moist Exposed Burn Treatment/Moist Ex-posed Burn Ointment ， MEBT/MEBO）对大鼠糖尿病足溃疡创面愈合的干预作用，研究其促进溃疡创面愈合的机制。方法将100只雄性SD大鼠随机分为空白对照组（20只）和糖尿病造模组（80只），糖尿病造模组大鼠尾静脉注射链脲佐菌素（STZ），成功制备糖尿病大鼠模型64只，从中随机选取60只，并随机分为湿润烧伤膏（MEBO）组（20只）、贝复济组（20只）和模型组（20只）。造模成功后次日开始用药，各组大鼠创面给予相应的药物治疗，并记录创面愈合时间；用药6 d后，采用RT-PCR法观察记录创面肉芽组织中β-catenin mRNA和Smad3 mRNA的表达量。结果（1）4组大鼠创面平均愈合时间对比，空白对照组愈合时间最短； MEBO组、贝复济组、模型组3组大鼠创面平均愈合时间对比， MEBO组愈合时间最短。（2）用药后第6天，模型组大鼠创面肉芽组织中β-catenin mRNA的表达显著低于空白对照组、 MEBO组、贝复济组（ P＜0.01），而MEBO 组与贝复济组相比，差异具有统计学意义（P＜0.05）；模型组大鼠创面肉芽组织中Smad3 mRNA的表达显著高于空白对照组、 MEBO组、贝复济组（P＜0.01），而MEBO组与贝复济组相比，差异具有统计学意义（ P＜0.05）。结论 MEBT/MEBO可抑制Smad3 mRNA、增强β-catenin mRNA在实验性糖尿病足溃疡大鼠创面修复中的表达，缩短创面愈合时间，促进糖尿病足溃疡创面的愈合。
목적：채용RT-PCR검측방법관찰피부원위재생복원기술（ Moist Exposed Burn Treatment/Moist Ex-posed Burn Ointment ， MEBT/MEBO）대대서당뇨병족궤양창면유합적간예작용，연구기촉진궤양창면유합적궤제。방법장100지웅성SD대서수궤분위공백대조조（20지）화당뇨병조모조（80지），당뇨병조모조대서미정맥주사련뇨좌균소（STZ），성공제비당뇨병대서모형64지，종중수궤선취60지，병수궤분위습윤소상고（MEBO）조（20지）、패복제조（20지）화모형조（20지）。조모성공후차일개시용약，각조대서창면급여상응적약물치료，병기록창면유합시간；용약6 d후，채용RT-PCR법관찰기록창면육아조직중β-catenin mRNA화Smad3 mRNA적표체량。결과（1）4조대서창면평균유합시간대비，공백대조조유합시간최단； MEBO조、패복제조、모형조3조대서창면평균유합시간대비， MEBO조유합시간최단。（2）용약후제6천，모형조대서창면육아조직중β-catenin mRNA적표체현저저우공백대조조、 MEBO조、패복제조（ P＜0.01），이MEBO 조여패복제조상비，차이구유통계학의의（P＜0.05）；모형조대서창면육아조직중Smad3 mRNA적표체현저고우공백대조조、 MEBO조、패복제조（P＜0.01），이MEBO조여패복제조상비，차이구유통계학의의（ P＜0.05）。결론 MEBT/MEBO가억제Smad3 mRNA、증강β-catenin mRNA재실험성당뇨병족궤양대서창면수복중적표체，축단창면유합시간，촉진당뇨병족궤양창면적유합。
O bjective To study the mechanism of MEBT/MEBO promoting the healing of diabetic foot ulcer ( DFU) wounds by inspecting the intervention of MEBT /MEBO in the wound healing of rat DFU through RT-PCR detection . Methods One hundred male SD rats were divided randomly into blank Control Group ( 20 ) and Diabetes Model Building Group (80), the latter one was injected with STZ in caudal vein , 64 rat models were successfully built , and 60 of them were randomly selected and divided into MEBO group (20), Rb-bFGF group (20) and model group (20).After the model was built , the medicine was administered the next day ,each group of rat wounds was given corresponding medical treatment and the healing time was recorded .Six days later, RT-PCR was adopted to detect and record the amount of β-catenin mRNA and Smad3 mRNA expression in granulation tissues of wounds .Results (1) Comparing the average healing time of the 4 groups of rats , the blank Control Group was the shortest;The average healing time for MEBO Group was the shortest com-pared with Rb-bFGF Group and Model Group .(2) Six days after drug administration , the expression of β-catenin mRNA in wound granulations of rats in Model Group was obviously lower than that of blank Control Group , MEBO Group , and Rb-bFGF Group ( P<0.01 ) , and comparison between MEBO Group and Rb-bFGF group showed difference of statistical significance (P<0.05);The expression of Smad3 mRNA in wound granulations of rats in Model Group was obviously high-er than blank Control Group, MEBO Group, and Rb-bFGF Group (P<0.01), and comparison between MEBO Group and Rb-bFGF group showed difference of statistical significance ( P<0.05) .Conclusion MEBT/MEBO can repress expres-sion of Smad3 mRNA and enhance expression of β-catenin mRNA in healing of experimental DFU wounds of rats , shortening wound healing time and promoting healing of DFU wounds .