CAJ | 학술논문

从绿豆中提取粗酶并优化，活性测定反应条件，结果表明，在40℃、pH=7的条件下，酶反应活性最高。将来源于绿豆的环氧水解酶固载于多种硅基介孔材料上，考察了多种因素对固定化酶活性的影响。研究发现，以大孔径、表面疏水性的材料作为载体，采用共价方法固载酶，可以得到较高的酶固载率，同时固载酶表现出较高的催化反应活性，并且可以循环使用多次。
종록두중제취조매병우화，활성측정반응조건，결과표명，재40℃、pH=7적조건하，매반응활성최고。장래원우록두적배양수해매고재우다충규기개공재료상，고찰료다충인소대고정화매활성적영향。연구발현，이대공경、표면소수성적재료작위재체，채용공개방법고재매，가이득도교고적매고재솔，동시고재매표현출교고적최화반응활성，병차가이순배사용다차。
In this paper, rude epoxide hydrolase was prepared from mung beans and then the re-action conditions were optimized. The results showed that the optimum temperature is 40℃ and the optimum pH is 7. 0. Then several kinds of mesoporous silicas (MPSs) were used to immobi-lize enzyme to enhance its chemical and mechanical stabilities. The results indicated that materi-als with large pore size, hydrophobic surface gave higher immobilization efficiency and activity. Furthermore, covalent method resulted in higher stability and the solid enzyme can be reused.