CAJ | 학술논문

目的：探索静电纺丝技术制备小口径聚乳酸-己内酯[P(LLA-CL)]/纤维蛋白原管形支架的方法，评价支架的生物相容性，探讨其作为血管组织工程材料的可行性。方法以P(LLA-CL)、纤维蛋白原为原料，制备小口径复合管形支架，观察支架的大体形态，并用扫描电镜观察三维结构；利用溶血试验、细胞毒性试验、皮下植入试验，评价支架材料的生物相容性。结果管形支架表面呈网格状三维结构，并有大小不等、互相交通的孔隙，孔径平均直径为（4.56±1.23）μm，表面纤维平均直径（318±56） nm；P(LLA-CL)/纤维蛋白原浸提液溶血率为2.87%±0.49%；细胞毒性实验示P(LLA-CL)/纤维蛋白原浸提液较阴性对照组无明显差异（P>0.05）；皮下植入试验显示P(LLA-CL)/纤维蛋白原支架炎症反应轻微，材料逐渐降解。结论通过静电纺丝技术可以构建小口径P(LLA-CL)/纤维蛋白原管形支架，并具有良好的生物相容性，可作为组织工程血管的支架材料。
목적：탐색정전방사기술제비소구경취유산-기내지[P(LLA-CL)]/섬유단백원관형지가적방법，평개지가적생물상용성，탐토기작위혈관조직공정재료적가행성。방법이P(LLA-CL)、섬유단백원위원료，제비소구경복합관형지가，관찰지가적대체형태，병용소묘전경관찰삼유결구；이용용혈시험、세포독성시험、피하식입시험，평개지가재료적생물상용성。결과관형지가표면정망격상삼유결구，병유대소불등、호상교통적공극，공경평균직경위（4.56±1.23）μm，표면섬유평균직경（318±56） nm；P(LLA-CL)/섬유단백원침제액용혈솔위2.87%±0.49%；세포독성실험시P(LLA-CL)/섬유단백원침제액교음성대조조무명현차이（P>0.05）；피하식입시험현시P(LLA-CL)/섬유단백원지가염증반응경미，재료축점강해。결론통과정전방사기술가이구건소구경P(LLA-CL)/섬유단백원관형지가，병구유량호적생물상용성，가작위조직공정혈관적지가재료。
Objective To fabricate a small diameter tubular scaffold of P (LLA-CL)/fibrinogen by electrospinning fiber technique, to evaluate the biocompatibility of the P (LLA-CL)/fibrinogen scaffold and its feasibility for vascular tissue engineering. Methods A small diameter tubular scaffold was fabricated by co-electrospinning blend of P (LLA-CL) and Fibrinogen. Gross morphology of the scaffold was observed and the 3-dimensional structure was observed by scanning electron microscope (SEM). Biocompatibilities of the tubular scaffold were evaluated in vivo and in vitro by acute hemolysis test, cytotoxicity test, and short-term test of subcutanous implantation. Results A randomly oriented nanofibrous structure with a well interconnected network of pores was observed. The diameter of the fiber at the outer surface was 318 ±56 nm and the average pore diameter was 4.56 ±1.23 μm; Hemolysis rate was 2.87%±0.49%; There was no significant difference of cytotoxicity between the P (LLA-CL)/fibrinogen tubular scaffold and negative control group (P>0.05). After the placement in rat subcutaneous pouches, the scaffolds were gradually biodegraded with little inflammatory reaction. Conclusion The P (LLA-CL)/fibrinogen tubular scaffold can be fabricated by electrospinning fiber technique. It has good biocompatibility and could be potentially used as vascular tissue engineering scaffold material.