中国中医药科技
中國中醫藥科技
중국중의약과기
CHINESE JOURNAL OF TRADITIONAL MEDICAL SCIENCE AND TECHNOLOGY
2014年
5期
522-523
,共2页
二脱甲氧基姜黄素%宫颈癌细胞Hela%Caspase-8
二脫甲氧基薑黃素%宮頸癌細胞Hela%Caspase-8
이탈갑양기강황소%궁경암세포Hela%Caspase-8
two demethoxylation curcumin%human cervical carcinoma cell Hera%Caspase -8
目的：观察二脱甲氧基姜黄素对体外培养人宫颈癌Hela细胞Caspase-8的作用。方法：MTT法检测二脱甲氧基姜黄素对体外培养人宫颈癌细胞Hela的抑制作用；分光光度计检测其对Caspase-8酶活性的影响；免疫组化法检测其对人宫颈癌细胞Hela Caspase-8蛋白表达的影响。结果：二脱甲氧基姜黄素对人宫颈癌细胞Hela有明显的抑制作用（ P＜0.001），且呈剂量关系，与姜黄素有显著差异（ P＜0.001）；二脱甲氧基姜黄素能增强Caspase-8酶活性，增强Caspase-8蛋白的表达。结论：二脱甲氧基姜黄素能激活Caspase-8蛋白活性，这可能也是其体外抑制宫颈癌细胞Hela生长的机理之一。
目的：觀察二脫甲氧基薑黃素對體外培養人宮頸癌Hela細胞Caspase-8的作用。方法：MTT法檢測二脫甲氧基薑黃素對體外培養人宮頸癌細胞Hela的抑製作用；分光光度計檢測其對Caspase-8酶活性的影響；免疫組化法檢測其對人宮頸癌細胞Hela Caspase-8蛋白錶達的影響。結果：二脫甲氧基薑黃素對人宮頸癌細胞Hela有明顯的抑製作用（ P＜0.001），且呈劑量關繫，與薑黃素有顯著差異（ P＜0.001）；二脫甲氧基薑黃素能增彊Caspase-8酶活性，增彊Caspase-8蛋白的錶達。結論：二脫甲氧基薑黃素能激活Caspase-8蛋白活性，這可能也是其體外抑製宮頸癌細胞Hela生長的機理之一。
목적：관찰이탈갑양기강황소대체외배양인궁경암Hela세포Caspase-8적작용。방법：MTT법검측이탈갑양기강황소대체외배양인궁경암세포Hela적억제작용；분광광도계검측기대Caspase-8매활성적영향；면역조화법검측기대인궁경암세포Hela Caspase-8단백표체적영향。결과：이탈갑양기강황소대인궁경암세포Hela유명현적억제작용（ P＜0.001），차정제량관계，여강황소유현저차이（ P＜0.001）；이탈갑양기강황소능증강Caspase-8매활성，증강Caspase-8단백적표체。결론：이탈갑양기강황소능격활Caspase-8단백활성，저가능야시기체외억제궁경암세포Hela생장적궤리지일。
Objective:To observe the effects of two demethoxylation curcumin on Caspase -8 in Hela cells cultured in vitro .Methods:MTT method was used to determine the inhibition effect of two demethoxylation cur-cumin on human cervical carcinoma cell line Hela cultured in vitro;Caspase -8 activity was detected by spec-trophotometry ;Caspase-8 protein expression of human cervical carcinoma cells Hela was determined by immu-nohistochemistry assay .Results: Two demethoxylation curcumin could significantly inhibit the proliferation of human cervical carcinoma cells Hela (P<0.01), the effect was taken shape of dose -dependentment relation-ship, there was obvious difference to compare with curcumin ( P<0.01);two demethoxylation curcumin could enhance Caspase-8 activity and the expression of Caspase -8 protein in Hela cells.Conclusion:Two deme-thoxylation curcumin could activate Caspase -8 protein, which may be one of the mechanism for inhibiting the proliferation of human cervical carcinoma cells Hela .
