CAJ | 학술논문

目的：观察绞股蓝总皂苷对高糖培养条件下小鼠永生足细胞nephrin、VEGF（ vascular endothelial growth factor ，血管内皮生长因子） mRNA表达的影响。方法：利用体外培养条件性永生小鼠足细胞，高糖干预24小时，模拟糖尿病肾病足细胞损伤，分为正常组、高糖模型组、高渗对照组、绞股蓝治疗组（绞股蓝总皂苷高、中、低剂量组）及缬沙坦对照组，Real Time PCR 法检测足细胞nephrin、VEGF mRNA的表达。结果：绞股蓝总皂苷干预组nephrin mRNA含量显著升高，VEGF mRNA表达有一定程度提高，与模型对照组比较，差异有显著统计学意义（ P＜0.05或P＜0.01）。结论：绞股蓝总皂苷可有效调节肾脏足细胞nephrin、VEGF mRNA表达。
목적：관찰교고람총조감대고당배양조건하소서영생족세포nephrin、VEGF（ vascular endothelial growth factor ，혈관내피생장인자） mRNA표체적영향。방법：이용체외배양조건성영생소서족세포，고당간예24소시，모의당뇨병신병족세포손상，분위정상조、고당모형조、고삼대조조、교고람치료조（교고람총조감고、중、저제량조）급힐사탄대조조，Real Time PCR 법검측족세포nephrin、VEGF mRNA적표체。결과：교고람총조감간예조nephrin mRNA함량현저승고，VEGF mRNA표체유일정정도제고，여모형대조조비교，차이유현저통계학의의（ P＜0.05혹P＜0.01）。결론：교고람총조감가유효조절신장족세포nephrin、VEGF mRNA표체。
Objective:To observe the effects of Gypenosides on nephrin , VEGFmRNA expressions of Mice's podocytes in hyperglycemia conditions .Methods: Mice’ s podocytes cultured by high glucose in vitro for 24 hours,to make podocyte injury model of diabetic nephropathy , experiment were divided into normal group , model group, high glucose control group , treatment group ( Gynostemma gypenosides high , middle, low dose group ) and valsartan group , the expressions of nephrin ,VEGF mRNA were determined by Real -Time PCR.Results:The expression of nephrin mRNA significantly increased in Gypenosides intervention group ,VEGFmRNA expres-sion was increased to a certain extent ,to compare with model group ,the difference was statistically significant ( P<0.05 or P<0.01 ).Conclusion: Gypenosides can regulate expressions of nephrin 、VEGF mRNA in renal podocyte .