中国中医药科技
中國中醫藥科技
중국중의약과기
CHINESE JOURNAL OF TRADITIONAL MEDICAL SCIENCE AND TECHNOLOGY
2014年
5期
494-496
,共3页
黎鹏程%卢丽丽%皮丕喆%陈聪%谭峰
黎鵬程%盧麗麗%皮丕喆%陳聰%譚峰
려붕정%로려려%피비철%진총%담봉
动脉粥样硬化%斑块稳定%舒心稳斑颗粒%MMP-9%I型胶原蛋白%兔
動脈粥樣硬化%斑塊穩定%舒心穩斑顆粒%MMP-9%I型膠原蛋白%兔
동맥죽양경화%반괴은정%서심은반과립%MMP-9%I형효원단백%토
atherosclerotic%plaques plaque stabilization%Shuxin wenban granule%MMP-9%COL-I%rabbit
目的：观察舒心稳斑颗粒对实验兔AS斑块稳定性及MMP-9、I型胶原蛋白表达的影响。方法：运用高脂高胆固醇饲料喂养12周制备日本大耳白兔动脉粥样硬化模型，用舒心稳斑颗粒灌胃给药治疗4周，采用双抗体夹心酶联免疫吸附法检测血清中I型胶原蛋白的含量；采用免疫组化法分析I型胶原蛋白、MMP-9在主动脉粥样斑块中的表达；同时观察主动脉粥样硬化斑块的病理学改变。结果：与模型组比较，舒心稳斑颗粒能升高血清中Ⅰ型胶原蛋白的含量，差异具有显著统计学意义（ P＜0.01）；同时能降低斑块中MMP-9的表达水平，升高Ⅰ型胶原蛋白在斑块中的表达水平。病理学检查结果显示，采用舒心稳斑颗粒干预后，主动脉粥样硬化斑块的病理学形态、结构均发生改变，斑块结构更加趋于稳定。结论：舒心稳斑颗粒可能通过抑制斑块内炎症反应，调节动脉粥样硬化斑块纤维帽中的胶原纤维降解与合成失衡状态，进而对AS斑块起稳定作用。
目的：觀察舒心穩斑顆粒對實驗兔AS斑塊穩定性及MMP-9、I型膠原蛋白錶達的影響。方法：運用高脂高膽固醇飼料餵養12週製備日本大耳白兔動脈粥樣硬化模型，用舒心穩斑顆粒灌胃給藥治療4週，採用雙抗體夾心酶聯免疫吸附法檢測血清中I型膠原蛋白的含量；採用免疫組化法分析I型膠原蛋白、MMP-9在主動脈粥樣斑塊中的錶達；同時觀察主動脈粥樣硬化斑塊的病理學改變。結果：與模型組比較，舒心穩斑顆粒能升高血清中Ⅰ型膠原蛋白的含量，差異具有顯著統計學意義（ P＜0.01）；同時能降低斑塊中MMP-9的錶達水平，升高Ⅰ型膠原蛋白在斑塊中的錶達水平。病理學檢查結果顯示，採用舒心穩斑顆粒榦預後，主動脈粥樣硬化斑塊的病理學形態、結構均髮生改變，斑塊結構更加趨于穩定。結論：舒心穩斑顆粒可能通過抑製斑塊內炎癥反應，調節動脈粥樣硬化斑塊纖維帽中的膠原纖維降解與閤成失衡狀態，進而對AS斑塊起穩定作用。
목적：관찰서심은반과립대실험토AS반괴은정성급MMP-9、I형효원단백표체적영향。방법：운용고지고담고순사료위양12주제비일본대이백토동맥죽양경화모형，용서심은반과립관위급약치료4주，채용쌍항체협심매련면역흡부법검측혈청중I형효원단백적함량；채용면역조화법분석I형효원단백、MMP-9재주동맥죽양반괴중적표체；동시관찰주동맥죽양경화반괴적병이학개변。결과：여모형조비교，서심은반과립능승고혈청중Ⅰ형효원단백적함량，차이구유현저통계학의의（ P＜0.01）；동시능강저반괴중MMP-9적표체수평，승고Ⅰ형효원단백재반괴중적표체수평。병이학검사결과현시，채용서심은반과립간예후，주동맥죽양경화반괴적병이학형태、결구균발생개변，반괴결구경가추우은정。결론：서심은반과립가능통과억제반괴내염증반응，조절동맥죽양경화반괴섬유모중적효원섬유강해여합성실형상태，진이대AS반괴기은정작용。
Objective:To observe the effects of Shuxin wenban granule on the stability of AS plaque and the expressions of MMP-9 and COL-l in rabbits.Methods: Using the high cholesterol diet to induce AS rabbit models for 12 weeks, then, giving Shuxin wenban granule for 4 weeks, finally,detecting the serum levels of COL-I in the rabbits by double antibody sandwich enzyme -linked immunosorbent method and the surface density of MMP-9 and COL-I in atherosclerotic plaques by immunohistochemical staining .At the same time , observing the pathological changes of aorta in atherosclerotic rabbits .Results:To compared with model group , Shuxin wen-ban granule could increase the serum levels of COL -I,the difference was statistically significant (P<0.01);similarly, Shuxin wenban granule could significantly decrease the surface density of MMP -9 and increase the surface density of COL-I in atherosclerotic plaques,the difference was statistically significant (P<0.01).Pa-thology showed that Shuxin wenban granule changed the pathological morphology , structure of atherosclerotic plaque , caused the plaque to stabler .Conclusion: Shuxin wenban granules could stabilize atherosclerotic plaques by inhibiting the inflammatory response of atherosclerotic plaque and affecting the imbalance of collagen fibers’ degradation and synthesis in atherosclerotic plaque fibrous cap .
