中国中医药科技
中國中醫藥科技
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CHINESE JOURNAL OF TRADITIONAL MEDICAL SCIENCE AND TECHNOLOGY
2014年
5期
479-481
,共3页
徐莉莉%宋晓琳%张露芬%朱江%任晓暄%李晓泓
徐莉莉%宋曉琳%張露芬%硃江%任曉暄%李曉泓
서리리%송효림%장로분%주강%임효훤%리효홍
痛经%镇痛效应%前列腺素 F2α%前列腺素E2%β-内啡肽%合谷穴%电针%大鼠
痛經%鎮痛效應%前列腺素 F2α%前列腺素E2%β-內啡肽%閤穀穴%電針%大鼠
통경%진통효응%전렬선소 F2α%전렬선소E2%β-내배태%합곡혈%전침%대서
dysmenorrheal%analgesic effect%PGF2α%PGE2%β-EP%Hegu point%electro-acupuncture%rat
目的：初步研究电针合谷穴对痛经大鼠的镇痛效应及机制。方法：将30只处于动情间期的SD雌性大鼠随机分为3组：正常组、模型组、电针合谷组。采用苯甲酸雌二醇和缩宫素联合制备痛经大鼠模型，观察电针合谷穴对痛经大鼠扭体行为、前列腺素F2α（ PGF2α）、前列腺素E2（ PGE2）、β-内啡肽（β-EP）含量的影响。结果：与模型组比较，电针合谷穴组大鼠扭体潜伏期明显延长，扭体次数、总分均显著性减少（均P＜0.01）；子宫PGE2含量无显著性差异（ P＞0.05）， PGF2α含量及PGF2α/PGE2比值均显著性下降（均P＜0.01）；血浆、子宫、下丘脑β-EP含量均无显著性差异（均P＞0.05），垂体β-EP显著性升高（ P＜0.01）。结论：电针合谷穴可以通过降低大鼠子宫PGF2α含量和PGF2α/PGE2比值、升高垂体β-EP含量达到止痛的作用，其镇痛效应机制可能是通过提高中枢β-EP 的含量达到的。
目的：初步研究電針閤穀穴對痛經大鼠的鎮痛效應及機製。方法：將30隻處于動情間期的SD雌性大鼠隨機分為3組：正常組、模型組、電針閤穀組。採用苯甲痠雌二醇和縮宮素聯閤製備痛經大鼠模型，觀察電針閤穀穴對痛經大鼠扭體行為、前列腺素F2α（ PGF2α）、前列腺素E2（ PGE2）、β-內啡肽（β-EP）含量的影響。結果：與模型組比較，電針閤穀穴組大鼠扭體潛伏期明顯延長，扭體次數、總分均顯著性減少（均P＜0.01）；子宮PGE2含量無顯著性差異（ P＞0.05）， PGF2α含量及PGF2α/PGE2比值均顯著性下降（均P＜0.01）；血漿、子宮、下丘腦β-EP含量均無顯著性差異（均P＞0.05），垂體β-EP顯著性升高（ P＜0.01）。結論：電針閤穀穴可以通過降低大鼠子宮PGF2α含量和PGF2α/PGE2比值、升高垂體β-EP含量達到止痛的作用，其鎮痛效應機製可能是通過提高中樞β-EP 的含量達到的。
목적：초보연구전침합곡혈대통경대서적진통효응급궤제。방법：장30지처우동정간기적SD자성대서수궤분위3조：정상조、모형조、전침합곡조。채용분갑산자이순화축궁소연합제비통경대서모형，관찰전침합곡혈대통경대서뉴체행위、전렬선소F2α（ PGF2α）、전렬선소E2（ PGE2）、β-내배태（β-EP）함량적영향。결과：여모형조비교，전침합곡혈조대서뉴체잠복기명현연장，뉴체차수、총분균현저성감소（균P＜0.01）；자궁PGE2함량무현저성차이（ P＞0.05）， PGF2α함량급PGF2α/PGE2비치균현저성하강（균P＜0.01）；혈장、자궁、하구뇌β-EP함량균무현저성차이（균P＞0.05），수체β-EP현저성승고（ P＜0.01）。결론：전침합곡혈가이통과강저대서자궁PGF2α함량화PGF2α/PGE2비치、승고수체β-EP함량체도지통적작용，기진통효응궤제가능시통과제고중추β-EP 적함량체도적。
Objective:To preliminarily study the analgesic effects of electroacupuncture ( EA ) at Hegu (LI4)point in dysmenorrhea rats and explore the mechanism .Methods:30 SD female rats aged three-month-old in the estrum were randomly divided into normal group, model group and EA group. Rats were given estradiol benzoate combined with oxytocin to prepare the models of dysmenorrhea and normal group was given the same dose of saline , EA group was given EA .The effect of EA at Hegu on the writhing behavior , PGE2、PGF2α、β-EP levels in dysmenorrheal rats were observed .Results:To compared with model group , the rats'writhing incuba-tion period in EA at LI4 group was obviously prolonged ,rats'writhing number and scores were decreased signifi-cantly(all P<0.01);there was no significant difference between the two groups on uterus PGE 2 level (P>0. 05);PGF2αlevel and PGF2α/PGE2 ratio were reduced significantly in EA group (all P<0.01);β-EP level in plasma,uterus and hypothalamus had no significant difference (all P>0.05), in pituitary that was significantly increased(P <0.01).Conclusions:Electro-acupuncture at Hegu point can reduce PGF 2αlevels and PGF2α/PGE2 ratio in the rats'uterus,increae β-EP level to achieve analgesic effect .
