CAJ | 학술논문

目的：了解儿童呼吸道流感嗜血杆菌的临床分布特征和耐药特点，为指导临床合理用药提供科学依据。方法收集儿童痰液标本进行培养并分离出流感嗜血杆菌，用K-B纸片扩散法进行抗菌药物敏感试验，并对其进行β内酰胺酶测定；数据用WHONET5．6统计软件统计分析。结果从12374份痰液标本中共分离出1256株流感嗜血杆菌，分离率为10．2％；1256株流感嗜血杆菌对氨苄西林、甲氧苄啶-磺胺甲口恶唑和氨苄西林-舒巴坦的耐药率分别为37．8％、65．5％和16．5％，对其余测试的抗菌药物耐药率均＜10．0％；β内酰胺酶阳性率为33．5％。结论流感嗜血杆菌对氨苄西林和甲氧苄啶-磺胺甲口恶唑的耐药性较高；该菌对于头孢克洛、氨苄西林-舒巴坦、头孢他啶、头孢克肟、阿奇霉素、环丙沙星、美罗培南、利福平的敏感率均在80．0％以上。产β内酰胺酶是流感嗜血杆菌对氨苄西林的主要耐药机制。
목적：료해인동호흡도류감기혈간균적림상분포특정화내약특점，위지도림상합리용약제공과학의거。방법수집인동담액표본진행배양병분리출류감기혈간균，용K-B지편확산법진행항균약물민감시험，병대기진행β내선알매측정；수거용WHONET5．6통계연건통계분석。결과종12374빈담액표본중공분리출1256주류감기혈간균，분리솔위10．2％；1256주류감기혈간균대안변서림、갑양변정-광알갑구악서화안변서림-서파탄적내약솔분별위37．8％、65．5％화16．5％，대기여측시적항균약물내약솔균＜10．0％；β내선알매양성솔위33．5％。결론류감기혈간균대안변서림화갑양변정-광알갑구악서적내약성교고；해균대우두포극락、안변서림-서파탄、두포타정、두포극우、아기매소、배병사성、미라배남、리복평적민감솔균재80．0％이상。산β내선알매시류감기혈간균대안변서림적주요내약궤제。
Objective To investigate the antibiotic resistance of Haemophilus influenzae isolates collected from the children with respiratory tract infection for rational use of antibiotics in clinical practice .Methods The H .influenzae strains were isolated from children and subjected to antimicrobial susceptibility testing by Kirby-Bauer method .Nitrocefin disc test was used to detect the production of beta-lactamases .WHONET 5 .6 software was used to analyze the susceptibility data .Results A totalof1256strainsof H.influenzaewereisolated.About37.8% ,65.5% and16.5% ofthe1256strainsof H.influenzae were resistant to ampicillin ,trimethoprim-sulfamethoxazole ,and ampicillin-sulbactam ,respectively .Less than 10 .0% of these strains were resistant to any other antibiotics tested .Beta-lactamase was produced in 33 .5% of the 1 256 strains of H . influenzae .Conclusions The H . influenzae strains in this study are mainly resistant ampicillin and trimethoprim-sulfamethoxazole .About 80 .0% of these H . influenzae strains were still susceptible to cefaclor ,ampicillin-sulbactam , cefixime ,ceftazidime ,azithromycin ,ciprofloxacin ,meropenem and rifampin .The primary mechanism of ampicillin resistance in Haemophilus is production of beta-lactamases .