听力学及言语疾病杂志
聽力學及言語疾病雜誌
은역학급언어질병잡지
JOURNAL OF AUDIOLOGY AND SPEECH PATHOLOGY
2014年
5期
507-509
,共3页
神经生长因子%丁胺卡那霉素%耳毒性%毛细胞
神經生長因子%丁胺卡那黴素%耳毒性%毛細胞
신경생장인자%정알잡나매소%이독성%모세포
Nerve growth factor%Amikacin%Ototoxicity%Hair cells
目的：探讨鼠神经生长因子（nerve growth factor ，NGF）对丁胺卡那霉素（amikacin ，AK）致豚鼠听力损伤的保护作用。方法45只豚鼠随机分为对照组、中毒组、治疗组三组，每组15只。对照组不予药物处理；中毒组腹腔注射AK400 mg · kg -1· d-1，连续10天；治疗组同法注射AK 的同时肌肉注射 NGF 1500 AU · kg -1· d-1，连续14天。各组于给药前1天、给药结束后第10、20天分别行ABR测试，最后一次测试结束后处死所有豚鼠并取出听泡，行基底膜硝酸银染色铺片，观察耳蜗毛细胞形态结构的变化及毛细胞缺失率。结果给药前三组豚鼠ABR反应阈差异无统计学意义（P＞0．05），给药结束后第10、20天中毒组ABR阈值均高于治疗组及对照组（P＜0．05），治疗组ABR阈值高于对照组（P＜0．05）。耳蜗基底膜硝酸银染色铺片显示对照组耳蜗基底膜内毛细胞（IHC）、外毛细胞（OHC）排列整齐、偶有缺失；中毒组OHC广泛缺失；治疗组毛细胞损伤相对较轻，毛细胞缺失率较中毒组低（P＜0．05）。结论鼠神经生长因子对丁胺卡那霉素所致听损伤有保护作用。
目的：探討鼠神經生長因子（nerve growth factor ，NGF）對丁胺卡那黴素（amikacin ，AK）緻豚鼠聽力損傷的保護作用。方法45隻豚鼠隨機分為對照組、中毒組、治療組三組，每組15隻。對照組不予藥物處理；中毒組腹腔註射AK400 mg · kg -1· d-1，連續10天；治療組同法註射AK 的同時肌肉註射 NGF 1500 AU · kg -1· d-1，連續14天。各組于給藥前1天、給藥結束後第10、20天分彆行ABR測試，最後一次測試結束後處死所有豚鼠併取齣聽泡，行基底膜硝痠銀染色鋪片，觀察耳蝸毛細胞形態結構的變化及毛細胞缺失率。結果給藥前三組豚鼠ABR反應閾差異無統計學意義（P＞0．05），給藥結束後第10、20天中毒組ABR閾值均高于治療組及對照組（P＜0．05），治療組ABR閾值高于對照組（P＜0．05）。耳蝸基底膜硝痠銀染色鋪片顯示對照組耳蝸基底膜內毛細胞（IHC）、外毛細胞（OHC）排列整齊、偶有缺失；中毒組OHC廣汎缺失；治療組毛細胞損傷相對較輕，毛細胞缺失率較中毒組低（P＜0．05）。結論鼠神經生長因子對丁胺卡那黴素所緻聽損傷有保護作用。
목적：탐토서신경생장인자（nerve growth factor ，NGF）대정알잡나매소（amikacin ，AK）치돈서은력손상적보호작용。방법45지돈서수궤분위대조조、중독조、치료조삼조，매조15지。대조조불여약물처리；중독조복강주사AK400 mg · kg -1· d-1，련속10천；치료조동법주사AK 적동시기육주사 NGF 1500 AU · kg -1· d-1，련속14천。각조우급약전1천、급약결속후제10、20천분별행ABR측시，최후일차측시결속후처사소유돈서병취출은포，행기저막초산은염색포편，관찰이와모세포형태결구적변화급모세포결실솔。결과급약전삼조돈서ABR반응역차이무통계학의의（P＞0．05），급약결속후제10、20천중독조ABR역치균고우치료조급대조조（P＜0．05），치료조ABR역치고우대조조（P＜0．05）。이와기저막초산은염색포편현시대조조이와기저막내모세포（IHC）、외모세포（OHC）배렬정제、우유결실；중독조OHC엄범결실；치료조모세포손상상대교경，모세포결실솔교중독조저（P＜0．05）。결론서신경생장인자대정알잡나매소소치은손상유보호작용。
Objective To investigate the effects of mouse nerve growth factor on amikacin (AK)-induced ototoxicity in guinea pigs .Methods 45 guinea pigs were randomly divided into three groups with 15 guinea pigs in each group .The control group received no drug treatment ,the poisoning group received an intraperitoneal injection of AK (400 mg · kg -1 · d-1 ) for 10 days ,and the treatment group received an intraperitoneal injection of AK (400 mg · kg -1 · d-1 ) for 10 days and an intramuscular injection of NGF (1 500 AU · kg -1 · d-1 ) for 14 consecutive days .Auditory brainstem responses were tested on the day before administration ,the 10th day and 20th day after administration .All the guinea pigs were sacrificed when the last ABR test was finished on the 20th day whose Corti organs were observed as well .Results The ABR thresholds of each group showed no significant difference before administration .The ABR threshold of the treatment group was lower than that of the poisoning group .With light microscopy ,the hair cells with normal morphology were observed in the control group .However ,there was an ex-tensive loss of outer hair cell in the poisoning group .For the treatment group ,the miss rate of hair cell was lower compared with the poisoning group .Conclusion Our data shows that NGF has played a protective role on amikacin-induced ototoxicity .
