CAJ | 학술논문

为了进一步提高产朊假丝酵母全细胞转化生物合成谷胱甘肽（ GSH）的能力，利用响应面分析方法对酵母培养的发酵培养基进行优化。在单因素实验的基础上，通过Plackett-Burman设计筛选出显著影响GSH转化力的2个主要因素：葡萄糖和KH2 PO4。采用最陡爬坡试验和响应面设计预测了葡萄糖和KH2 PO4的最佳质量浓度分别为58?5和17?2 g/L。验证实验结果表明，在该优化培养基条件下，酵母细胞的GSH转化力为1?54 mg/（ g·h），比优化前提高了1倍。该结果为类似的采用全细胞转化法高效合成有用化学品的研究与开发提供了可行的优化思路。
위료진일보제고산원가사효모전세포전화생물합성곡광감태（ GSH）적능력，이용향응면분석방법대효모배양적발효배양기진행우화。재단인소실험적기출상，통과Plackett-Burman설계사선출현저영향GSH전화력적2개주요인소：포도당화KH2 PO4。채용최두파파시험화향응면설계예측료포도당화KH2 PO4적최가질량농도분별위58?5화17?2 g/L。험증실험결과표명，재해우화배양기조건하，효모세포적GSH전화력위1?54 mg/（ g·h），비우화전제고료1배。해결과위유사적채용전세포전화법고효합성유용화학품적연구여개발제공료가행적우화사로。
Candida utilis SZU 07-01 was used as a whole-cell catalyst for the production of glutathione ( GSH) by bioconversion. In order to improve the capability of the yeast cell on GSH biotransformation,the response surface methodology was applied for the media optimization. Based on the results of the single-factor experiments together with the Plackett-Burman design, glucose and KH2 PO4 were selected as the primary factors with significant influences on GSH biotransformation rate. The optimal concentrations of 58?5 and 17?2 g/L for glucose and KH2 PO4 were predicted by using the steepest ascent test and the response surface methodology,respectively?According to the verification experiments in flasks,the average GSH biotransformation rate of 1?54 mg/( g·h ) was achieved under the optimized medium, which was increased by 100%compared with that without media optimization. The results provided a feasible approach on the efficient production of analogical useful chemicals synthesized by the whole-cell bioconversion.