CAJ | 학술논문

以外消旋4氯3羟基丁酸乙酯为唯一C源的富集培养筛选得到一株菌株WZ009，经16S rDNA测序鉴定为巨大芽胞杆菌（ Bacillus megaterium）。 B?megaterium WZ009静息细胞可以立体选择性催化（ S）4氯3羟基丁酸乙酯水解和脱氯反应得到光学纯的（ R）4氯3羟基丁酸乙酯（ e?e?≥99％）和（ S）3羟基γ丁内酯（ e?e?≥95％）。笔者对B?megaterium WZ009不对称催化反应影响因素（温度、pH、中和剂、底物浓度、时间进程以及细胞重复利用）进行优化研究，确定了该反应体系最优条件：底物浓度200 mmol/L，中和剂氨水，pH 7?2，40℃反应12 h，转化率达到50?6％，底物对映体过量值为99?6％。该生物催化合成（ R）4氯3羟基丁酸乙酯和（ S）3羟基γ丁内酯过程具有良好的工业化应用前景。
이외소선4록3간기정산을지위유일C원적부집배양사선득도일주균주WZ009，경16S rDNA측서감정위거대아포간균（ Bacillus megaterium）。 B?megaterium WZ009정식세포가이입체선택성최화（ S）4록3간기정산을지수해화탈록반응득도광학순적（ R）4록3간기정산을지（ e?e?≥99％）화（ S）3간기γ정내지（ e?e?≥95％）。필자대B?megaterium WZ009불대칭최화반응영향인소（온도、pH、중화제、저물농도、시간진정이급세포중복이용）진행우화연구，학정료해반응체계최우조건：저물농도200 mmol/L，중화제안수，pH 7?2，40℃반응12 h，전화솔체도50?6％，저물대영체과량치위99?6％。해생물최화합성（ R）4록3간기정산을지화（ S）3간기γ정내지과정구유량호적공업화응용전경。
A new strain WZ009 was isolated by enrichment culture with ethyl-4-chloro-3-hydroxybutyrate as the only source of carbon?Strain WZ009 was identified as Bacillus megaterium by 16S rDNA gene sequencing. The resting cell of B?megaterium WZ009 catalyzed stereoselective hydrolysis and dechlorination of ethyl-(S)-4-chloro-3-hydroxybutyrate,obtained high optical active ethyl-(R)-4-chloro-3-hydroxybutyrate ( e?e? ≥99%) and ( S )-3-hydroxy-γ-butyrolactone ( e?e? ≥95%) . The catalytic characteristics of B?megaterium WZ009 were studied. The optimal conditions were obtained as follows:substrate concentration 200 mmol/L,reaction temperature 40 ℃,pH 7?2, neutralizing agent NH3·H2 O and reaction time 12 h. Under the above-mentioned conditions, the conversion was 50?6% with e?e?99?6%. The biocatalysis synthesis of ethyl-( R )-4-chloro-3-hydroxybutyrate and ( S )-3-hydroxy-γ-butyrolactone has a bright industrialization future.