CAJ | 학술논문

酶是参与植物体内生化反应的特殊蛋白质。在保持活组织和细胞结构完整性的条件下，利用组织化学、细胞化学、免疫学和显微检测等技术研究酶的即位定位，是了解酶在组织、细胞和亚细胞中的分布、活性动态与定量及酶功能等的重要途径。对植物体中酶定位的组织化学和细胞化学方法的概念、原理与研究进展进行了综述，并根据国际酶化学分类编号顺序，分别介绍了25种酶的组织化学染色定位所用的反应介质和染色方法及46种酶的细胞化学定位方法的参考文献。
매시삼여식물체내생화반응적특수단백질。재보지활조직화세포결구완정성적조건하，이용조직화학、세포화학、면역학화현미검측등기술연구매적즉위정위，시료해매재조직、세포화아세포중적분포、활성동태여정량급매공능등적중요도경。대식물체중매정위적조직화학화세포화학방법적개념、원리여연구진전진행료종술，병근거국제매화학분류편호순서，분별개소료25충매적조직화학염색정위소용적반응개질화염색방법급46충매적세포화학정위방법적삼고문헌。
Enzymes are special proteins that catalyze and regulate a series of life processes in plants. Studies on in situ histochemical, cytochemical, immunological and microscopical localizations of metabolism enzymes in intact plant tissues and cells are important approaches in understanding the distributions, activity dynamics and quantiifcations, and functions of enzymes within tissues, cells and organelles. The basic concepts, principles and research progresses of histochemical and cytochemical localizations of enzymes were reviewed. According to the international enzymology classiifcation and taxis, the reaction media and staining method of histochemical localizations on 25 plant enzymes and references of cytochemical localizations on 46 plant enzymes were introduced, respectively.