CAJ | 학술논문

目的：研究不同浓度的川芎嗪注射液（TMP）对体外人精子运动参数的影响，并初步探讨其可能的作用机制。方法20例健康生育男性手淫供精，将上游优化处理的精子，与不同浓度TMP注射液共同孵育10、30、60min，采用计算机辅助精子分析系统观察不同浓度TMP（0.1、1、10mmol/L）体外对精子各运动参数的作用。结果与对照组相比，1mmol/L浓度组，除60min时精子活率[（a+b+c）级精子%]未下降外，其余时间精子活率、前向运动精子百分率[（a+b）级精子%]、平均直线速度（VSL）和平均路径速度（VAP）等观察指标均明显下降，差异均具统计学意义（P＜0.05，P＜0.01）。10mmol/L浓度组，精子活率、前向运动精子百分率、VSL和VAP等观察指标也均明显下降，差异均有统计学意义（P＜0.01）。与对照组相比，侧摆幅度（ALH）除0.1mmol/L浓度组和1mmol/L浓度组30min时未下降外，余均有下降，但差异无统计学意义（P＞0.05）。结论川芎嗪体外能显著降低精子运动能力，为男性节育提供了一种新选择。
목적：연구불동농도적천궁진주사액（TMP）대체외인정자운동삼수적영향，병초보탐토기가능적작용궤제。방법20례건강생육남성수음공정，장상유우화처리적정자，여불동농도TMP주사액공동부육10、30、60min，채용계산궤보조정자분석계통관찰불동농도TMP（0.1、1、10mmol/L）체외대정자각운동삼수적작용。결과여대조조상비，1mmol/L농도조，제60min시정자활솔[（a+b+c）급정자%]미하강외，기여시간정자활솔、전향운동정자백분솔[（a+b）급정자%]、평균직선속도（VSL）화평균로경속도（VAP）등관찰지표균명현하강，차이균구통계학의의（P＜0.05，P＜0.01）。10mmol/L농도조，정자활솔、전향운동정자백분솔、VSL화VAP등관찰지표야균명현하강，차이균유통계학의의（P＜0.01）。여대조조상비，측파폭도（ALH）제0.1mmol/L농도조화1mmol/L농도조30min시미하강외，여균유하강，단차이무통계학의의（P＞0.05）。결론천궁진체외능현저강저정자운동능력，위남성절육제공료일충신선택。
Objective To investigate the effects of the Tetramethylpyrazine (TMP) on human sperm motility in vitro and further explore its possible mechanisms. Methods Sperm samples from 20 healthy fertile men were collected by masturbation, and then they were treated by swim-up technique to produce a spermatozoa solution of high motility. Subsequently they were incubated with different concentration of tetramethylpyrazine solution( 0.1, 1, 10mmol/L) invitro. Sperm motility parameters at incubation time of 10, 30 and 60min were measured by computer-aided sperm analysis (CASA). Results Contrast to the control group, In group 1mmol/L and 10 mmol/L,TMP markedly decreased the sperm live rate, progressive motility, VSL, VAP, there were significant difference statistically contrast to the control group. But ALH had no significant difference statistically contrast to the control group. Conclusion The data suggest that tetramethylpyrazine can inhibit sperm motility in vitro, which may be a new approach for male contraception.