中国男科学杂志
中國男科學雜誌
중국남과학잡지
CHINESE JOURNAL OF ANDROLOGY
2014年
8期
9-12
,共4页
彭玉婉%程龙飞%贺小进%杨晓玉%王晶%魏兆莲%周平%宋兵%王彬彬%马旭%曹云霞
彭玉婉%程龍飛%賀小進%楊曉玉%王晶%魏兆蓮%週平%宋兵%王彬彬%馬旭%曹雲霞
팽옥완%정룡비%하소진%양효옥%왕정%위조련%주평%송병%왕빈빈%마욱%조운하
输精管/畸形%囊性纤维化跨膜转导调节因子%基因
輸精管/畸形%囊性纖維化跨膜轉導調節因子%基因
수정관/기형%낭성섬유화과막전도조절인자%기인
vas deferens/abnormalities%cystic fibrosis transmembrane conductance regulator%genes
目的：研究非电压门控钠离子通道基因SCNN1G以及IL1B基因与先天性双侧输精管缺如（CBAVD）间的关系。方法通过直接测序和聚合酶链反应-限制性片段长度多态性（PCR-RELP）方法检测基因多态性位点rs5735（SCNN1G基因）和rs3917356（IL1B基因）在109例病例和103例对照人群中的基因型频率的分布。结果两个单核苷酸多态性（SNP）位点在病例-对照组中的等位基因、基因型频率分布差异无显著性（P=0.2410, P=0.1701 rs5735；P=0.5564, P=0.0959 rs3917356）结论 SCNN1G基因（rs5735）、IL1B基因（rs3917356）均未发现与CBAVD之间存在明显的关联性。
目的：研究非電壓門控鈉離子通道基因SCNN1G以及IL1B基因與先天性雙側輸精管缺如（CBAVD）間的關繫。方法通過直接測序和聚閤酶鏈反應-限製性片段長度多態性（PCR-RELP）方法檢測基因多態性位點rs5735（SCNN1G基因）和rs3917356（IL1B基因）在109例病例和103例對照人群中的基因型頻率的分佈。結果兩箇單覈苷痠多態性（SNP）位點在病例-對照組中的等位基因、基因型頻率分佈差異無顯著性（P=0.2410, P=0.1701 rs5735；P=0.5564, P=0.0959 rs3917356）結論 SCNN1G基因（rs5735）、IL1B基因（rs3917356）均未髮現與CBAVD之間存在明顯的關聯性。
목적：연구비전압문공납리자통도기인SCNN1G이급IL1B기인여선천성쌍측수정관결여（CBAVD）간적관계。방법통과직접측서화취합매련반응-한제성편단장도다태성（PCR-RELP）방법검측기인다태성위점rs5735（SCNN1G기인）화rs3917356（IL1B기인）재109례병례화103례대조인군중적기인형빈솔적분포。결과량개단핵감산다태성（SNP）위점재병례-대조조중적등위기인、기인형빈솔분포차이무현저성（P=0.2410, P=0.1701 rs5735；P=0.5564, P=0.0959 rs3917356）결론 SCNN1G기인（rs5735）、IL1B기인（rs3917356）균미발현여CBAVD지간존재명현적관련성。
Objective To investigate whether SCNN1G and IL-1β polymorphisms were associated with the development of congenital bilateral absence of the vas deferens (CBAVD). Methods A total of 222 Han Chinese males, including 109 CBAVD and 103 controls were recruited in the study. IL-1B (rs3917356) and sodium channel non-voltage-gated 1 (SCNN1G, encoding the ENaC γ-subunit) (rs5735) polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct sequencing. Results No significant differences in gene locus frequency of either SNP were found between patients with CBAVD and the control (P=0.2410, P=0.1701 rs5735;P=0.5564, P=0.0959 rs3917356, respectively). Conclusion These results suggest that SNPs of SCNNIG gene and IL1B gene may not be associated with the development of CBAVD.
