CAJ | 학술논문

目的：探讨mirR-361-5p在激素非依赖性前列腺癌细胞PC3中的表达和作用。方法采用qRT-PCR检测转染后的PC3中miRNA-361-5p的表达情况，用CCK-8和克隆形成实验检测转染后的PC3细胞增殖能力，流式细胞仪检测细胞周期和细胞凋亡的变化，Transwell实验检测细胞侵袭能力。结果 qRT-PCR检测结果显示PC3中miR-361-5p含量低，脂质体法有较高的转染效率。CCK-8与克隆形成实验表明在PC3中升高miR-361-5p能够抑制前列腺癌细胞的活性和增殖能力。流式细胞检测发现miR-361-5p可增加细胞阻滞在G0/G1期和凋亡的比例。Transwell实验中，转染miR-361-5p后细胞通过小室的数量明显减少。结论 miR-361-5p能够抑制PC3的增殖与侵袭能力，从而发挥抑癌的作用。
목적：탐토mirR-361-5p재격소비의뢰성전렬선암세포PC3중적표체화작용。방법채용qRT-PCR검측전염후적PC3중miRNA-361-5p적표체정황，용CCK-8화극륭형성실험검측전염후적PC3세포증식능력，류식세포의검측세포주기화세포조망적변화，Transwell실험검측세포침습능력。결과 qRT-PCR검측결과현시PC3중miR-361-5p함량저，지질체법유교고적전염효솔。CCK-8여극륭형성실험표명재PC3중승고miR-361-5p능구억제전렬선암세포적활성화증식능력。류식세포검측발현miR-361-5p가증가세포조체재G0/G1기화조망적비례。Transwell실험중，전염miR-361-5p후세포통과소실적수량명현감소。결론 miR-361-5p능구억제PC3적증식여침습능력，종이발휘억암적작용。
Objective To investigate the effects of mirR-361-5p on bilogical behaviors of androgen-independent human prostate cancer cellsPC3. Methods The expression of miR-361-5p in PC3 cells after transfection was detected by qRT-PCR. Cell viability and proliferation of PC3 cells trasfected with miR-361-5p were measured by Cell Counting Kit-8(CCK-8) assay and colony formation assay. Cell cycle and apoptosis of PC3 cells transfected with miR-361-5p were analyzed by flow cytometry. The invasion ability of cell was evaluated by Transwell assay. Results The results of qRT-PCR showed that the expression of miR-361-5p in PC3 cells was low, indicating transfection efficiency was higher using liposome method. Overexpression of miR-361-5p significantly inhibited the proliferation of PC3 cells. Cell cycle analysis of PC3 cells transfected with miR-361-5p showed that cell number in G0/G1 phase was high and cell apoptosis number was low. MiR-361-5p significantly inhibited the number of cells migrated through Transwell chambers membrane. Conclusion These findings suggest that miR-361-5p may be a tumor suppressor miR in PC3 and it can inhibit the proliferation and invasion ability of androgen-independent human prostate cancer cells.