CAJ | 학술논문

背景：原发性胆汁性肝硬化（ PBC）是一种自身免疫性肝病，目前发病机制尚未完全阐明。除遗传和环境因素外，自身抗体、多种免疫细胞以及细胞因子共同参与了PBC的发生。近年研究发现白细胞介素-9（IL-9）具有多向免疫调节功能，与多种变态反应性疾病和自身免疫性疾病相关。目的：探讨IL-9在PBC患者中的表达及其临床意义。方法：收集PBC患者血清标本30例，肝穿刺标本20例，另取10名同期健康体检者外周血标本和4例正常肝组织标本作为正常对照组。采用ELISA法检测血清IL-9水平，采用免疫组化染色检测肝组织IL-9表达。分析IL-9水平与血清生化、免疫指标和肝组织病理分期的相关性。结果：PBC患者血清IL-9水平较正常对照组显著升高（ P<0.05），并与血清IgG水平呈正相关（r=0.681，P<0.01）。PBC患者肝组织中的IL-9阳性细胞数量较正常对照组显著增加（P<0.01），并与肝组织病理分期呈正相关（rs =0.465，P<0.05）。结论：PBC患者外周血和肝组织中的IL-9均呈高表达，且其表达水平与血清IgG水平和肝组织病理分期相关，提示IL-9可能在PBC的发病过程中发挥重要作用。
배경：원발성담즙성간경화（ PBC）시일충자신면역성간병，목전발병궤제상미완전천명。제유전화배경인소외，자신항체、다충면역세포이급세포인자공동삼여료PBC적발생。근년연구발현백세포개소-9（IL-9）구유다향면역조절공능，여다충변태반응성질병화자신면역성질병상관。목적：탐토IL-9재PBC환자중적표체급기림상의의。방법：수집PBC환자혈청표본30례，간천자표본20례，령취10명동기건강체검자외주혈표본화4례정상간조직표본작위정상대조조。채용ELISA법검측혈청IL-9수평，채용면역조화염색검측간조직IL-9표체。분석IL-9수평여혈청생화、면역지표화간조직병리분기적상관성。결과：PBC환자혈청IL-9수평교정상대조조현저승고（ P<0.05），병여혈청IgG수평정정상관（r=0.681，P<0.01）。PBC환자간조직중적IL-9양성세포수량교정상대조조현저증가（P<0.01），병여간조직병리분기정정상관（rs =0.465，P<0.05）。결론：PBC환자외주혈화간조직중적IL-9균정고표체，차기표체수평여혈청IgG수평화간조직병리분기상관，제시IL-9가능재PBC적발병과정중발휘중요작용。
Background:Primary biliary cirrhosis( PBC ) is an autoimmune liver disease,the cause of the disease remain incompletely understood. In addition to genetic and environmental factors,autoantibodies,multiple immunocytes and cytokines are considered to be involved in the development of PBC. Recent studies indicated that interleukin-9(IL-9)had pleiotropic functions in inflammatory regulation in allergic and autoimmune diseases. Aims:To investigate the expression and clinical significance of IL-9 in patients with PBC. Methods:A total of 30 specimens of peripheral blood and 20 specimens of liver tissue from PBC patients were collected. Ten specimens of peripheral blood from healthy subjects and 4 specimens of normal liver tissue were served as controls. Level of serum IL-9 was determined by ELISA,and expression of IL-9 in liver tissue was detected by immunohistochemistry. Correlations between IL-9 and serum biochemical indicators, immune indicators and histologic stage of liver tissue were analyzed. Results:Level of serum IL-9 was significantly increased in PBC patients than in normal control group(P<0. 05),and was positively correlated with level of serum IgG (r=0. 681,P<0. 01). Amount of IL-9 positive cells in liver tissue was significantly increased in PBC patients than in normal control group(P <0. 01),and was positively correlated with histologic stage of liver tissue(rs =0. 465,P <0. 05). Conclusions:Expression of IL-9 is significantly increased in peripheral blood and liver tissue in patients with PBC and is positively correlated with level of serum IgG and histologic stage of liver tissue,which suggests an important role of IL-9 in the pathogenesis of PBC.