CAJ | 학술논문

用迭代目标转换因子分析法(ITTFA)辅助分光光度法不经分离同时测定了模拟样品和生化试剂实际样品中四环素、氯霉素和盐酸万古霉素的含量。当测量波长范围为(248~260) nm，波长点数为13，波长间隔为1 nm，主因子数为3时计算结果最佳。模拟样中3组分的平均测出率均接近100%，生化试剂实际样品中3组分的加标回收率在96%~105%。与高效液相色谱法相比，本文方法简便快速，无需分离和添加任何分析试剂，只需对试样进行紫外-可见吸收光谱扫描，即可利用 ITTFA 法消除共存的其他未知组分的干扰，完成对试样中指定的多种抗生素的同时测定。
용질대목표전환인자분석법(ITTFA)보조분광광도법불경분리동시측정료모의양품화생화시제실제양품중사배소、록매소화염산만고매소적함량。당측량파장범위위(248~260) nm，파장점수위13，파장간격위1 nm，주인자수위3시계산결과최가。모의양중3조분적평균측출솔균접근100%，생화시제실제양품중3조분적가표회수솔재96%~105%。여고효액상색보법상비，본문방법간편쾌속，무수분리화첨가임하분석시제，지수대시양진행자외-가견흡수광보소묘，즉가이용 ITTFA 법소제공존적기타미지조분적간우，완성대시양중지정적다충항생소적동시측정。
Iterative target transformation factor analysis (ITTFA) algorithm was used to assist spectrophotometric determination of TC, CAP and vancomycin hydrochloride in simulated samples and biochemical reagent samples simultaneously without separation. The best result was available while the absorbance at 13 wavelength points with interval of 1 nm in the range from (248 to 260) nm was selected in calculation, and the best main factor was 3. The mean recovery rate was nearly 100%for the three components in simulated samples, and it was 96%~105%in biochemical reagent samples. Compared to HPLC, spectrophotometry with ITTFA was simple without using any reagent and separation. Specified antibiotics in samples with coexisting unknown components could be determined conveniently according to the scanning data of UV-vis spectra.