CAJ | 학술논문

建立了一种利用间接ELISA检测花生中黄曲霉毒素B1的方法,同时考察了平衡相对湿度及贮存时间对花生中黄曲霉毒素B1( AFB1)含量的影响。结果表明：该方法的重现性好,AFB1回收率在95.20%~97.75%之间,线性范围为0.049~0.971μg/kg,最低检出限为0.029μg/kg；花生中AFB1的含量随平衡相对湿度的升高而增加,随贮存时间延长,其含量也不断增加。但平衡相对湿度低于57.6%时,即使贮存90 d,花生中的AFB1含量仍低于国家食品安全标准限量(20μg/kg)。
건립료일충이용간접ELISA검측화생중황곡매독소B1적방법,동시고찰료평형상대습도급저존시간대화생중황곡매독소B1( AFB1)함량적영향。결과표명：해방법적중현성호,AFB1회수솔재95.20%~97.75%지간,선성범위위0.049~0.971μg/kg,최저검출한위0.029μg/kg；화생중AFB1적함량수평형상대습도적승고이증가,수저존시간연장,기함량야불단증가。단평형상대습도저우57.6%시,즉사저존90 d,화생중적AFB1함량잉저우국가식품안전표준한량(20μg/kg)。
The method for determing aflatoxin B1 ( AFB1 ) in peanut by indirect ELISA was established,and the effects of equilibrium relative humidity and storage time on the content of AFB1 were studied. The re-sults showed that the method had good reproducibility, and the recovery rate, the linear range and the limit of detection were 95 . 20% -97 . 75%, 0 . 049 -0 . 971 μg/kg and 0 . 029 μg/kg respectively; the content of AFB1 in peanut increased with equilibrium relative humidity rising and storage time prolonging, while the content of AFB1 was still below 20 μg/kg of the limitation of the national food safety standard, when peanut was stored for 90 d under the condition of the equilibrium relative humidity below 57 . 6%.