CAJ | 학술논문

将人工合成带有生物素标记的禽流感病毒两条多肽与亲和素结合，形成亲和素-生物素-合成肽连接物，然后免疫小鼠。采用酶联免疫吸附试验进行抗体检测。检测结果发现这些连接物能够诱导抗亲和素抗体的产生，但不能诱导抗合成肽抗体的产生，检测结果表明亲和素不能借助其与生物素的特异性结合，成为合成肽疫苗的理想载体。
장인공합성대유생물소표기적금류감병독량조다태여친화소결합，형성친화소-생물소-합성태련접물，연후면역소서。채용매련면역흡부시험진행항체검측。검측결과발현저사련접물능구유도항친화소항체적산생，단불능유도항합성태항체적산생，검측결과표명친화소불능차조기여생물소적특이성결합，성위합성태역묘적이상재체。
In this study,two peptides labeled of avian inlfuenza virus with biotin were synthesized,and mixed with avidin to form avidin - biotin - peptide complexes,and used to immunize mice. Antibody was detected by enzyme linked immunosorbent assay(ELISA). The detection results showed that these complexes could induce anti-avidin antibodies,but they couldn’t induce antibodies against the synthesized peptide,suggesting that avidin cannot be considered as an ideal carrier of synthetic peptide vaccine through its specific binding to biotin.