中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2014年
9期
1698-1702
,共5页
谢英花%马燕山%张楠%张建新
謝英花%馬燕山%張楠%張建新
사영화%마연산%장남%장건신
硫化氢%急性心肌缺血%大鼠%心脏%NF-κB%炎症
硫化氫%急性心肌缺血%大鼠%心髒%NF-κB%炎癥
류화경%급성심기결혈%대서%심장%NF-κB%염증
Hydrogen sulfide%Acute myocardial ischemia%Rats%Heart%NF-kappa B%Inflammation
目的：探讨硫化氢（ H2 S）能否抑制大鼠急性心肌缺血引起的细胞炎症反应。方法：结扎大鼠左冠状动脉前降支4 h，引起心肌缺血损伤。健康雄性SD大鼠随机分为假手术组（ sham）、缺血组（ ischemia）以及硫氢化钠（ NaHS）5μmol/L、10μmol/L和20μmol/L 组。 NaHS 组分别于心肌急性缺血2 h时更换为5μmol/L、10μmol/L和20μmol/L NaHS灌流液。缺血后4 h记录心功能变化。实时荧光定量PCR检测离体心肌组织中TNF-α、IL-1β、IL-6、IL-10和ICAM-1 mRNA表达；Western blotting 检测离体心肌组织中NF-κB的表达。结果： Ischemia组离体心肌功能下降（与sham组相比P＜0.01），NaHS组离体心肌功能比ischemia组明显改善（P＜0.05或P＜0.01）。 Ischemia组离体心肌组织中TNF-α、IL-1β、IL-6和ICAM-1 mRNA表达显著增强，IL-10 mRNA表达显著降低（与sham组相比P＜0.01）；NaHS组离体心肌组织中TNF-α、IL-1β、IL-6和ICAM-1 mRNA表达比ischemia组明显降低，NaHS 10μmol/L，20μmol/L组离体心肌组织中IL-10 mRNA表达比ischemia组明显升高（ P＜0.05或P＜0.01）。与sham组相比，ischemia组NF-κB表达显著增加（P＜0.01），而NaHS 10μmol/L和20μmol/L组NF-κB的表达明显低于ischemia组（P＜0.05或P＜0.01）。结论： H2S可通过阻断NF-κB相关信号通路的转导，抑制炎症反应，明显改善大鼠急性心肌缺血损伤。
目的：探討硫化氫（ H2 S）能否抑製大鼠急性心肌缺血引起的細胞炎癥反應。方法：結扎大鼠左冠狀動脈前降支4 h，引起心肌缺血損傷。健康雄性SD大鼠隨機分為假手術組（ sham）、缺血組（ ischemia）以及硫氫化鈉（ NaHS）5μmol/L、10μmol/L和20μmol/L 組。 NaHS 組分彆于心肌急性缺血2 h時更換為5μmol/L、10μmol/L和20μmol/L NaHS灌流液。缺血後4 h記錄心功能變化。實時熒光定量PCR檢測離體心肌組織中TNF-α、IL-1β、IL-6、IL-10和ICAM-1 mRNA錶達；Western blotting 檢測離體心肌組織中NF-κB的錶達。結果： Ischemia組離體心肌功能下降（與sham組相比P＜0.01），NaHS組離體心肌功能比ischemia組明顯改善（P＜0.05或P＜0.01）。 Ischemia組離體心肌組織中TNF-α、IL-1β、IL-6和ICAM-1 mRNA錶達顯著增彊，IL-10 mRNA錶達顯著降低（與sham組相比P＜0.01）；NaHS組離體心肌組織中TNF-α、IL-1β、IL-6和ICAM-1 mRNA錶達比ischemia組明顯降低，NaHS 10μmol/L，20μmol/L組離體心肌組織中IL-10 mRNA錶達比ischemia組明顯升高（ P＜0.05或P＜0.01）。與sham組相比，ischemia組NF-κB錶達顯著增加（P＜0.01），而NaHS 10μmol/L和20μmol/L組NF-κB的錶達明顯低于ischemia組（P＜0.05或P＜0.01）。結論： H2S可通過阻斷NF-κB相關信號通路的轉導，抑製炎癥反應，明顯改善大鼠急性心肌缺血損傷。
목적：탐토류화경（ H2 S）능부억제대서급성심기결혈인기적세포염증반응。방법：결찰대서좌관상동맥전강지4 h，인기심기결혈손상。건강웅성SD대서수궤분위가수술조（ sham）、결혈조（ ischemia）이급류경화납（ NaHS）5μmol/L、10μmol/L화20μmol/L 조。 NaHS 조분별우심기급성결혈2 h시경환위5μmol/L、10μmol/L화20μmol/L NaHS관류액。결혈후4 h기록심공능변화。실시형광정량PCR검측리체심기조직중TNF-α、IL-1β、IL-6、IL-10화ICAM-1 mRNA표체；Western blotting 검측리체심기조직중NF-κB적표체。결과： Ischemia조리체심기공능하강（여sham조상비P＜0.01），NaHS조리체심기공능비ischemia조명현개선（P＜0.05혹P＜0.01）。 Ischemia조리체심기조직중TNF-α、IL-1β、IL-6화ICAM-1 mRNA표체현저증강，IL-10 mRNA표체현저강저（여sham조상비P＜0.01）；NaHS조리체심기조직중TNF-α、IL-1β、IL-6화ICAM-1 mRNA표체비ischemia조명현강저，NaHS 10μmol/L，20μmol/L조리체심기조직중IL-10 mRNA표체비ischemia조명현승고（ P＜0.05혹P＜0.01）。여sham조상비，ischemia조NF-κB표체현저증가（P＜0.01），이NaHS 10μmol/L화20μmol/L조NF-κB적표체명현저우ischemia조（P＜0.05혹P＜0.01）。결론： H2S가통과조단NF-κB상관신호통로적전도，억제염증반응，명현개선대서급성심기결혈손상。
AIM:To investigate whether hydrogen sulfide ( H2 S) protects the hearts against inflammatory re-sponses induced by acute myocardial ischemia in isolated rat hearts .METHODS: Rat acute myocardial ischemia injury was induced by ligation of the left anterior descending coronary artery for 4 h, and the normal perfusate was replaced with NaHS (5 μmol/L, 10μmol/L and 20 μmol/L) perfusate accordingly in NaHS groups 2 h after ischemia.The changes of cardiac function in the myocardial ischemic injury rats were observed .The mRNA expression of TNF-α, IL-1β, IL-6, IL-10 and ICAM-1 was detected by real-time PCR.The protein level of nuclear factor-κB ( NF-κB) in the myocardial tissues was detected by Western blotting .RESULTS:The cardiac function in ischemia group was lower than that in sham group (P<0.01).Compared with ischemia group, perfusion of NaHS resulted in the improvement of the cardiac function (P<0.05 or P<0.01).Compared with sham group, the mRNA expression of TNF-α, IL-1β, IL-6 and ICAM-1 in the cardiac tissues was significantly increased , and the mRNA expression of IL-10 in the cardiac tissues was significantly decreased in ischemia group (P<0.01).Compared with ischemia group , the perfusion of NaHS significantly decreased the mRNA ex-pression of TNF-α, IL-1β, IL-6 and ICAM-1 ( P<0.05 or P<0.01 ) .The perfusion of NaHS at concentrations of 10μmol/L and 20μmol/L significantly increased the mRNA expression of IL-10 (P<0.01).The protein level of NF-κB in ischemia group was markedly higher than that in sham group (P<0.01).Compared with ischemia group, the perfusion of NaHS at concentrations of 10 μmol/L and 20 μmol/L significantly decreased the expression of NF-κB ( P<0.05 or P<0.01).CONCLUSION:H2S protects the hearts against acute ischemia injury through inhibition of NF-κB activation and subsequent down-regulation of NF-κB-dependent inflammatory gene expression .
